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| Product Name | Recombinant SARS-CoV-2 Nucleoprotein (N), P13L, Full Length, Active |
| Catalog No. | PTR-HMM-0233 |
| Description | A full-length recombinant SARS-CoV-2 nucleoprotein carrying the P13L substitution, expressed in E. coli with an N-terminal 6xHis tag. Binding to two nucleoprotein-specific antibodies was verified by functional ELISA. |
| Intended Use | Suitable for functional ELISA, antibody-binding studies, SARS-CoV-2 nucleocapsid variant research, and assay development. |
| Principle / Technology | A defined recombinant protein region is expressed and purified, then its structural quality and biological function are evaluated using the listed biochemical binding or activity assays. |
| Detection Method | SDS-PAGE; functional ELISA |
| Sample Type | Purified recombinant protein |
| Performance Range / Specifications | Purity: >=93% by SDS-PAGE. Molecular weight: 49.7 kDa. Package sizes: 10 ug; 20 ug; 50 ug; 100 ug; 200 ug; 500 ug; 1 mg. |
| Sensitivity / LOD | Functional ELISA EC50: 3.246-4.503 ng/mL with a mouse monoclonal antibody and 2.825-4.124 ng/mL with a recombinant antibody. |
| Specificity | Binding activity was demonstrated with two SARS-CoV-2 nucleoprotein-specific antibodies. |
| Reaction Conditions / Protocol | Functional ELISA showed binding to a mouse monoclonal nucleoprotein antibody with an EC50 of 3.246-4.503 ng/mL and to a recombinant nucleoprotein antibody with an EC50 of 2.825-4.124 ng/mL. |
| Components / Formulation | 20 mM Tris-HCl, 0.5 M NaCl, 6% trehalose, pH 8.0 |
| Storage Conditions | Store at -20 C or -80 C upon receipt. Aliquot before repeated use and avoid repeated freeze-thaw cycles. |
| Shelf Life | Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C. |
| Package Specifications | 10 ug; 20 ug; 50 ug; 100 ug; 200 ug; 500 ug; 1 mg |
| Product Form | Lyophilized powder |
| Quality Control | Purity evaluated by SDS-PAGE; biological activity verified by functional ELISA. |
| Key Features | Active full-length SARS-CoV-2 nucleoprotein; P13L variant; N-terminal 6xHis tag; E. coli expression. |
| Purity | >=93% by SDS-PAGE. |
| Activity / Unit Definition | Functional ELISA showed binding to a mouse monoclonal nucleoprotein antibody with an EC50 of 3.246-4.503 ng/mL and to a recombinant nucleoprotein antibody with an EC50 of 2.825-4.124 ng/mL. |
| Molecular Weight | 49.7 kDa |
| Source / Origin | SARS-CoV-2 protein produced in E. coli. |
| pH Range / Optimal pH | pH 8.0 |
| Expiration Date / Stability | Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C. |
| Regulatory / Compliance | For research use only; not for clinical diagnosis or treatment. |
| Compatibility | Suitable for functional ELISA and SARS-CoV-2 nucleoprotein antibody-binding studies. |
| Recommended Buffer System | 20 mM Tris-HCl, 0.5 M NaCl, 6% trehalose, pH 8.0 |
| Application Notes / Precautions | Briefly centrifuge the vial before opening. Reconstitute with sterile deionized water to 0.1-1.0 mg/mL. For long-term storage, add 5-50% glycerol as appropriate, divide into aliquots, and freeze. Avoid repeated freezing and thawing. A working aliquot may be kept at 4 C for up to one week. |
| Genotype | P13L |
| Alternative Names / Synonyms | Nucleoprotein; Nucleocapsid protein; NC; Protein N |
| Lead Time | 3-7 business days |
| Availability Status | In Stock |
| Host Species | SARS-CoV-2 |
| Tag Information | N-terminal 6xHis tag |
| Amino Acid Sequence | MSDNGPQNQRNALRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA |
| UniProt Accession | YP_009724397.1 |
| Protein Length | 419 aa |
| Expression Region | 1-419 aa (P13L) |
For research use only, not for clinical use.
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