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| Product Name | Recombinant SARS-CoV-2 Nucleoprotein (N), Full-Length, Active |
| Catalog No. | PTR-HMM-0162 |
| Description | Full-length SARS-CoV-2 nucleoprotein produced in E. coli with an N-terminal 6xHis tag. The 419-amino-acid construct is supplied as an active lyophilized protein for research applications. |
| Intended Use | Functional ELISA, antibody-binding studies, antigen research, and assay development. |
| Principle / Technology | A defined recombinant protein region is expressed and purified, then its structural integrity and target-binding function are evaluated using the listed biochemical binding assays. |
| Detection Method | SDS-PAGE; functional ELISA |
| Sample Type | Purified recombinant protein |
| Performance Range / Specifications | >=85% by SDS-PAGE; calculated molecular weight 49.7 kDa; package options: 100 ug; 1 mg. |
| Sensitivity / LOD | In functional ELISA, immobilized nucleoprotein at 2 ug/mL bound two independent anti-nucleoprotein antibodies with EC50 values of 1.368-1.804 ng/mL and 4.267-5.568 ng/mL. |
| Specificity | Binds antibodies directed against the SARS-CoV-2 nucleoprotein. |
| Reaction Conditions / Protocol | Briefly centrifuge the vial before opening. Reconstitute with sterile deionized water to 0.1-1.0 mg/mL. For long-term storage, add 5-50% glycerol as appropriate, divide into aliquots, and freeze. |
| Components / Formulation | 20 mM Tris-HCl, 0.5 M NaCl, and 6% trehalose, pH 8.0 |
| Storage Conditions | Store at -20 C or -80 C upon receipt. Aliquot before repeated use and avoid repeated freeze-thaw cycles. |
| Shelf Life | Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C. |
| Package Specifications | 100 ug; 1 mg |
| Product Form | Lyophilized powder |
| Quality Control | Purity evaluated by SDS-PAGE; endotoxin tested by LAL assay; biological activity verified by functional ELISA. |
| Key Features | Active SARS-CoV-2 recombinant protein; 1-419 aa expression region; E. coli expression; N-terminal 6xHis tag; >=85% by SDS-PAGE; low endotoxin. |
| Purity | >=85% by SDS-PAGE |
| Activity / Unit Definition | In functional ELISA, immobilized nucleoprotein at 2 ug/mL bound two independent anti-nucleoprotein antibodies with EC50 values of 1.368-1.804 ng/mL and 4.267-5.568 ng/mL. |
| Molecular Weight | 49.7 kDa |
| Source / Origin | SARS-CoV-2 protein produced in E. coli. |
| pH Range / Optimal pH | 8.0 |
| Expiration Date / Stability | Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C. |
| Regulatory / Compliance | For research use only; not for clinical diagnosis or treatment. |
| Compatibility | Functional ELISA, antibody-binding studies, antigen research, and assay development. |
| Recommended Buffer System | 20 mM Tris-HCl, 0.5 M NaCl, and 6% trehalose, pH 8.0 |
| Application Notes / Precautions | Avoid repeated freezing and thawing. A working aliquot may be kept at 4 C for up to one week. |
| Alternative Names / Synonyms | N; Nucleoprotein; Nucleocapsid protein; NC; Protein N |
| Lead Time | 3-7 business days |
| Availability Status | In Stock |
| Host Species | SARS-CoV-2 |
| Tag Information | N-terminal 6xHis tag |
| Amino Acid Sequence | MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTAAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA |
| UniProt Accession | YP_009724397.1 |
| Protein Length | 419 amino acids |
| Expression Region | 1-419 aa |
| Endotoxin Level | <1.0 EU/ug by LAL assay |
For research use only, not for clinical use.
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