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| Product Name | Recombinant Rat Microtubule-Associated Protein Tau (MAPT), Active |
| Catalog No. | PTR-HMM-0282 |
| Description | An active full-length recombinant mature protein of rat microtubule-associated protein tau, covering residues 2-752, produced in mammalian cells with n-terminal 10xhis tag. The preparation is designed for reproducible protein-binding and assay-development studies. |
| Intended Use | Suitable for Mapt antibody-binding studies, functional assays, target research, and assay development. |
| Principle / Technology | The specified rat Mapt region is expressed in mammalian cells, purified, and evaluated through functional binding or activity testing. |
| Detection Method | SDS-PAGE; functional ELISA; LAL assay |
| Sample Type | Purified recombinant protein |
| Performance Range / Specifications | Purity: >=95% by SDS-PAGE. EC50: 136.2-238.9 ng/mL. Molecular weight: 81.2 kDa. Package sizes: 10 ug; 20 ug; 50 ug; 100 ug; 200 ug; 500 ug; 1 mg. |
| Sensitivity / LOD | EC50: 136.2-238.9 ng/mL. |
| Specificity | Binding activity was demonstrated with a MAPT-specific recombinant antibody. |
| Reaction Conditions / Protocol | In a functional ELISA, immobilized rat MAPT at 2 ug/mL bound a MAPT-specific recombinant antibody with an EC50 of 136. |
| Components / Formulation | 20 mM Tris-HCl, 0.5 M NaCl, 6% trehalose, pH 8.0 |
| Storage Conditions | Store at -20 C or -80 C upon receipt. Aliquot before repeated use and avoid repeated freeze-thaw cycles. |
| Shelf Life | Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C. |
| Package Specifications | 10 ug; 20 ug; 50 ug; 100 ug; 200 ug; 500 ug; 1 mg |
| Product Form | Lyophilized powder |
| Quality Control | Purity evaluated by SDS-PAGE; endotoxin tested by LAL assay; biological activity validated by functional testing. |
| Key Features | Active rat Mapt; residues 2-752 aa; N-terminal 10xHis tag; mammalian cells; low endotoxin. |
| Purity | >=95% by SDS-PAGE. |
| Activity / Unit Definition | In a functional ELISA, immobilized rat MAPT at 2 ug/mL bound a MAPT-specific recombinant antibody with an EC50 of 136.2-238.9 ng/mL. |
| Molecular Weight | 81.2 kDa |
| Source / Origin | Rat protein produced in Mammalian cells. |
| pH Range / Optimal pH | pH 8.0 |
| Expiration Date / Stability | Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C. |
| Regulatory / Compliance | For research use only; not for clinical diagnosis or treatment. |
| Compatibility | Suitable for functional assays and Mapt-focused protein interaction studies. |
| Recommended Buffer System | 20 mM Tris-HCl, 0.5 M NaCl, 6% trehalose, pH 8.0 |
| Application Notes / Precautions | Briefly centrifuge the vial before opening. Reconstitute with sterile deionized water to 0.1-1.0 mg/mL when applicable. For long-term storage, add 5-50% glycerol as appropriate, divide into aliquots, and freeze. Avoid repeated freezing and thawing. A working aliquot may be kept at 4 C for up to one week. |
| Alternative Names / Synonyms | Neurofibrillary tangle protein; Paired helical filament tau; PHF-tau |
| Lead Time | 3-7 business days |
| Availability Status | In Stock |
| Host Species | Rat |
| Tag Information | N-terminal 10xHis tag |
| Amino Acid Sequence | AEPRQEFDTMEDQAGDYTMLQDQEGDMDHGLKESPPQPPADDGSEEPGSETSDAKSTPTAEDVTAPLVEERAPDKQATAQSHTEIPEGTTAEEAGIGDTPNMEDQAAGHVTQEPQKVEIFSQSLLVEPGRREGQAPDSGISDWTHQQVPSMSGAPLPPQGLREATHQPLGTRPEDVERSHPASELLWQESPQKEAWGKDRLGSEEEVDEDITMDESSQESPPSQASLAPGTATPQARSVSASGVSGETTSIPGFPAEGSIPLPADFFSKVSAETQASPPEGPGTGPSEEGHEAAPEFTFHVEIKASAPKEQDLEGATVVGAPAEEQKARGPSVGKGTKEASLLEPTDKQPAAGLPGRPVSRVPQLKARVAGVSKDRTGNDEKKAKTSTPSCAKTPSNRPCLSPTRPTPGSSDPLIKPSSPAVCPEPATSPKYVSSVTPRNGSPGTKQMKLKGADGKTGAKIATPRGAATPGQKGTSNATRIPAKTTPSPKTPPGSGEPPKSGERSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSASKSRLQTAPVPMPDLKNVRSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVHIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL |
| UniProt Accession | P19332 |
| Protein Length | 751 aa |
| Expression Region | 2-752 aa |
| Endotoxin Level | <1.0 EU/ug by the LAL method. |
For research use only, not for clinical use.
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