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| Product Name | Recombinant Protein G Sepharose Fast Flow Resin |
| Catalog No. | PTR-HMM-0086 |
| Description | A chromatography resin composed of recombinant Protein G ligand covalently coupled to cross-linked 4% agarose beads. This high-flow resin is designed for high-throughput antibody purification from cell culture harvest and biological fluids. Recombinant Protein G binds strongly to the Fc region of IgG from a broad range of mammalian species. The resin is supplied as a 50% slurry in 20% ethanol for direct packing into columns of any size. |
| Intended Use | Affinity capture of IgG antibodies from complex biological mixtures for laboratory-scale and pilot-scale purification. |
| Principle / Technology | Affinity chromatography with immobilized recombinant streptococcal Protein G |
| Detection Method | UV absorbance monitoring at 280 nm |
| Sample Type | Cell culture supernatant, serum, ascites, or partially purified antibody fractions |
| Performance Range / Specifications | Dynamic binding capacity: ≥25 mg human IgG per mL resin at 150 cm/hr linear flow; flow rate range: 50–400 cm/hr |
| Sensitivity / LOD | Purity of eluted IgG: ≥95% as determined by SDS-PAGE and size-exclusion HPLC |
| Specificity | Binds IgG1–IgG4 subclasses; strong affinity for rabbit, goat, sheep, cow, and horse IgG; weaker binding to mouse IgG1 compared to Protein A |
| Reaction Conditions / Protocol | Equilibration in 20 mM sodium phosphate, pH 7.0; load sample at 1–3 min residence time; wash with 10 CV equilibration buffer; elute with 0.1 M glycine-HCl, pH 2.7 |
| Components / Formulation | Recombinant Protein G Sepharose Fast Flow resin as 50% slurry in 20% ethanol |
| Storage Conditions | 2–8 °C; do not freeze |
| Shelf Life | 24 months |
| Package Specifications | 10 mL, 25 mL, 100 mL resin slurry volumes |
| Product Form | Bead suspension in preservative solution |
| Quality Control | Binding capacity verified per batch with human polyclonal IgG; ligand leaching below 10 ng/mg IgG eluted |
| Key Features | High cross-linked agarose matrix withstands linear flow rates up to 400 cm/hr for rapid processing |
| Purity | As specified per product type; recombinant proteins ≥90% by SDS-PAGE |
| Concentration | As specified on product label or certificate of analysis |
| Activity / Unit Definition | Binding capacity or enzymatic activity as specified per product documentation |
| Molecular Weight | Varies by product as specified in documentation |
| Source / Origin | Recombinant expression in E. coli, mammalian, or insect cell systems; synthetic chemistry for small molecule reagents |
| pH Range / Optimal pH | pH 7.0–8.0 for most protein applications |
| Shipping Conditions | Ambient for dry reagents; cold packs for liquid protein solutions; dry ice for frozen products |
| Expiration Date / Stability | 12–24 months at recommended storage temperature |
| Regulatory / Compliance | Research use; manufactured under ISO 9001 quality system |
| Compatibility | Compatible with standard SDS-PAGE, Western blot, mass spectrometry, and chromatography systems |
| Recommended Buffer System | PBS, Tris, or HEPES buffers as specified in product protocol |
| Application Notes / Precautions | Avoid repeated freeze-thaw cycles for protein solutions; use fresh aliquots; protect light-sensitive reagents |
| Batch-to-Batch Consistency | Protein concentration and activity within ±15% of reference lot |
For research use only, not for clinical use.
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