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| Product Name | Recombinant Mouse Microtubule-Associated Protein Tau (MAPT), Active |
| Catalog No. | PTR-HMM-0273 |
| Description | An active recombinant mouse tau protein representing residues 2-733 of the mature protein, expressed in mammalian cells with an N-terminal 10xHis tag. The purified protein retains antibody-binding activity in a functional ELISA. |
| Intended Use | Suitable for tau antibody development, functional ELISA, neurodegeneration research, and microtubule-associated protein studies. |
| Principle / Technology | The mature mouse MAPT sequence is expressed in mammalian cells, purified, and evaluated for antibody-binding activity by functional ELISA. |
| Detection Method | SDS-PAGE; functional ELISA; LAL assay |
| Sample Type | Purified recombinant protein |
| Performance Range / Specifications | Purity: >=90% by SDS-PAGE. EC50: 436.1-518.6 ng/mL. Molecular weight: 78.9 kDa. Package sizes: 20 ug; 100 ug; 1 mg. |
| Sensitivity / LOD | EC50: 436.1-518.6 ng/mL. |
| Specificity | Binding activity was demonstrated with a MAPT-specific recombinant antibody. |
| Reaction Conditions / Protocol | Immobilize mouse MAPT at 2 ug/mL and measure binding to a MAPT-specific recombinant antibody by functional ELISA. |
| Components / Formulation | 20 mM Tris-HCl, 0.5 M NaCl, 6% trehalose, pH 8.0 |
| Storage Conditions | Store at -20 C or -80 C upon receipt. Aliquot before repeated use and avoid repeated freeze-thaw cycles. |
| Shelf Life | Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C. |
| Package Specifications | 20 ug; 100 ug; 1 mg |
| Product Form | Lyophilized powder |
| Quality Control | Purity evaluated by SDS-PAGE; endotoxin tested by LAL assay; biological activity validated by functional testing. |
| Key Features | Active mouse tau; mature residues 2-733; N-terminal 10xHis tag; >=90% purity; mammalian expression; low endotoxin. |
| Purity | >=90% by SDS-PAGE. |
| Activity / Unit Definition | In a functional ELISA, immobilized mouse MAPT at 2 ug/mL bound a MAPT-specific recombinant antibody with an EC50 of 436.1-518.6 ng/mL. |
| Molecular Weight | 78.9 kDa |
| Source / Origin | Mouse protein produced in Mammalian cells. |
| pH Range / Optimal pH | pH 8.0 |
| Expiration Date / Stability | Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C. |
| Regulatory / Compliance | For research use only; not for clinical diagnosis or treatment. |
| Compatibility | Suitable for functional ELISA, tau antibody studies, and mouse neurobiology research. |
| Recommended Buffer System | 20 mM Tris-HCl, 0.5 M NaCl, 6% trehalose, pH 8.0 |
| Application Notes / Precautions | Briefly centrifuge the vial before opening. Reconstitute with sterile deionized water to 0.1-1.0 mg/mL when applicable. For long-term storage, add 5-50% glycerol as appropriate, divide into aliquots, and freeze. Avoid repeated freezing and thawing. A working aliquot may be kept at 4 C for up to one week. |
| Alternative Names / Synonyms | Neurofibrillary tangle protein; Paired helical filament tau; PHF-tau |
| Lead Time | 3-7 business days |
| Availability Status | In Stock |
| Host Species | Mouse |
| Tag Information | N-terminal 10xHis tag |
| Amino Acid Sequence | ADPRQEFDTMEDHAGDYTLLQDQEGDMDHGLKESPPQPPADDGAEEPGSETSDAKSTPTAEDVTAPLVDERAPDKQAAAQPHTEIPEGITAEEAGIGDTPNQEDQAAGHVTQGRREGQAPDLGTSDWTRQQVSSMSGAPLLPQGLREATCQPSGTRPEDIEKSHPASELLRRGPPQKEGWGQDRLGSEEEVDEDLTVDESSQDSPPSQASLTPGRAAPQAGSGSVCGETASVPGLPTEGSVPLPADFFSKVSAETQASQPEGPGTGPMEEGHEAAPEFTFHVEIKASTPKEQDLEGATVVGVPGEEQKAQTQGPSVGKGTKEASLQEPPGKQPAAGLPGRPVSRVPQLKARVASKDRTGNDEKKAKTSTPSCAKAPSHRPCLSPTRPTLGSSDPLIKPSSPAVSPEPATSPKHVSSVTPRNGSPGTKQMKLKGADGKTGAKIATPRGAASPAQKGTSNATRIPAKTTPSPKTPPGSGEPPKSGERSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSASKSRLQTAPVPMPDLKNVRSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL |
| UniProt Accession | P10637 |
| Protein Length | 732 aa |
| Expression Region | 2-733 aa |
| Endotoxin Level | <1.0 EU/ug by the LAL method. |
For research use only, not for clinical use.
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