Recombinant Masked Palm Civet Angiotensin-Converting Enzyme 2 (ACE2), Partial, Active

Name: Recombinant Masked Palm Civet Angiotensin-Converting Enzyme 2 (ACE2), Partial, Active
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Cat.No: PTR-HMM-0196 Datasheet

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Product Name	Recombinant Masked Palm Civet Angiotensin-Converting Enzyme 2 (ACE2), Partial, Active
Catalog No.	PTR-HMM-0196
Description	A recombinant masked palm civet ACE2 extracellular region spanning residues 18-740, produced in mammalian cells with a C-terminal human Fc tag. Both SARS-CoV RBD binding and fluorogenic substrate cleavage were verified.
Intended Use	Suitable for functional ELISA, fluorogenic ACE2 enzyme assays, and SARS-CoV RBD interaction studies.
Principle / Technology	A defined recombinant protein region is expressed and purified, then its structural quality and biological function are evaluated using the listed biochemical binding or activity assays.
Detection Method	SDS-PAGE; SEC-HPLC; functional ELISA; fluorogenic substrate assay; LAL assay
Sample Type	Purified recombinant protein
Performance Range / Specifications	Purity: >=90% as determined by SDS-PAGE and >=90% as determined by SEC-HPLC. Molecular weight: 112.7 kDa. Package sizes: 20 ug; 100 ug; 1 mg.
Sensitivity / LOD	Functional ELISA showed binding to immobilized SARS-CoV RBD at 2 ug/mL with an EC50 of 5.056-7.559 ng/mL. Enzyme activity measured with Mca-Ala-Pro-Lys(Dnp)-OH gave a Km of 22.84 uM.
Specificity	Binding activity was demonstrated with SARS-CoV receptor-binding domain; catalytic activity was demonstrated with Mca-Ala-Pro-Lys(Dnp)-OH.
Reaction Conditions / Protocol	Functional ELISA showed binding to immobilized SARS-CoV RBD at 2 ug/mL with an EC50 of 5.056-7.559 ng/mL. Enzyme activity measured with Mca-Ala-Pro-Lys(Dnp)-OH gave a Km of 22.84 uM.
Components / Formulation	PBS, 6% trehalose, pH 7.4
Storage Conditions	Store at -20 C or -80 C upon receipt. Aliquot before repeated use and avoid repeated freeze-thaw cycles.
Shelf Life	Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C.
Package Specifications	20 ug; 100 ug; 1 mg
Product Form	Lyophilized powder
Quality Control	Purity evaluated by SDS-PAGE and SEC-HPLC; endotoxin tested by LAL assay; biological activity verified by functional ELISA and a fluorogenic substrate assay.
Key Features	Active recombinant protein; 18-740 aa; C-terminal hFc1 tag; Mammalian cell.
Purity	>=90% as determined by SDS-PAGE and >=90% as determined by SEC-HPLC.
Activity / Unit Definition	Functional ELISA showed binding to immobilized SARS-CoV RBD at 2 ug/mL with an EC50 of 5.056-7.559 ng/mL. Enzyme activity measured with Mca-Ala-Pro-Lys(Dnp)-OH gave a Km of 22.84 uM.
Molecular Weight	112.7 kDa
Source / Origin	Paguma larvata (Masked palm civet) protein produced in Mammalian cell.
pH Range / Optimal pH	pH 7.4
Expiration Date / Stability	Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C.
Regulatory / Compliance	For research use only; not for clinical diagnosis or treatment.
Compatibility	Suitable for functional ELISA, fluorogenic ACE2 enzyme assays, and SARS-CoV RBD interaction studies.
Recommended Buffer System	PBS, 6% trehalose, pH 7.4
Application Notes / Precautions	Briefly centrifuge the vial before opening. Reconstitute with sterile deionized water to 0.1-1.0 mg/mL. For long-term storage, add 5-50% glycerol as appropriate, divide into aliquots, and freeze. Avoid repeated freezing and thawing. A working aliquot may be kept at 4 C for up to one week.
Alternative Names / Synonyms	ACE-related carboxypeptidase
Lead Time	3-7 business days
Availability Status	In Stock
Host Species	Paguma larvata (Masked palm civet)
Tag Information	C-terminal hFc1 tag
Amino Acid Sequence	QSTTEELAKTFLETFNYEAQELSYQSSVASWNYNTNITDENAKNMNEAGAKWSAYYEEQSKLAQTYPLAEIQDAKIKRQLQALQQSGSSVLSADKSQRLNTILNAMSTIYSTGKACNPNNPQECLLLEPGLDNIMENSKDYNERLWAWEGWRAEVGKQLRPLYEEYVALKNEMARANNYEDYGDYWRGDYEEEWTGGYNYSRNQLIQDVEDTFEQIKPLYQHLHAYVRAKLMDTYPSRISRTGCLPAHLLGDMWGRFWTNLYPLTVPFGQKPNIDVTDAMVNQNWDARRIFKEAEKFFVSVGLPNMTQGFWENSMLTEPGDGRKVVCHPTAWDLGKGDFRIKMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPNHLKTIGLLSPAFSEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGAIPKEQWMQKWWEMKRNIVGVVEPVPHDETYCDPASLFHVANDYSFIRYYTRTIYQFQFQEALCQIAKHEGPLHKCDISNSTEAGKKLLEMLSLGRSEPWTLALERVVGAKNMNVTPLLNYFEPLFTWLKEQNRNSFVGWDTDWRPYSDQSIKVRISLKSALGEKAYEWNDNEMYLFRSSIAYAMREYFSKVKNQTIPFVEDNVWVSDLKPRISFNFFVTFSNNVSDVIPRSEVEDAIRMSRSRINDAFRLDDNSLEFLGIEPTLSPPYRPPVT
UniProt Accession	Q56NL1
Protein Length	723 aa
Expression Region	18-740 aa
Endotoxin Level	<1.0 EU/ug as determined by the LAL method.

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For research use only, not for clinical use.