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| Product Name | Recombinant Human Pro-Neuregulin-1 (NRG1), Isoform 6, Partial, Active |
| Catalog No. | PTR-HMM-0205 |
| Description | A recombinant human NRG1 isoform 6 region spanning residues 20-247, produced in mammalian cells with a C-terminal 6xHis tag. ERBB3 binding and MCF-7 proliferation activity were verified. |
| Intended Use | Suitable for functional ELISA, ERBB3-binding studies, and MCF-7 cell proliferation assays. |
| Principle / Technology | A defined recombinant protein region is expressed and purified, then its structural quality and biological function are evaluated using the listed biochemical binding or activity assays. |
| Detection Method | SDS-PAGE; SEC-HPLC; functional ELISA; cell proliferation assay; LAL assay |
| Sample Type | Purified recombinant protein |
| Performance Range / Specifications | Purity: >=95% as determined by SDS-PAGE; >=95% as determined by SEC-HPLC. Molecular weight: 27.1 kDa. Package sizes: 20 ug; 100 ug; 1 mg. |
| Sensitivity / LOD | Functional ELISA showed binding to human ERBB3 with an EC50 of 18.24-23.66 ng/mL. The ED50 for dose-dependent stimulation of MCF-7 cell proliferation was 2.626-7.598 ng/mL. |
| Specificity | Biological activity was demonstrated through ERBB3 binding and dose-dependent MCF-7 cell proliferation. |
| Reaction Conditions / Protocol | Functional ELISA showed binding to human ERBB3 with an EC50 of 18.24-23.66 ng/mL. The ED50 for dose-dependent stimulation of MCF-7 cell proliferation was 2.626-7.598 ng/mL. |
| Components / Formulation | 20 mM Tris-HCl, 0.5 M NaCl, 6% trehalose, pH 8.0 |
| Storage Conditions | Store at -20 C or -80 C upon receipt. Aliquot before repeated use and avoid repeated freeze-thaw cycles. |
| Shelf Life | Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C. |
| Package Specifications | 20 ug; 100 ug; 1 mg |
| Product Form | Lyophilized powder |
| Quality Control | Purity evaluated by SDS-PAGE and SEC-HPLC; endotoxin tested by LAL assay; activity verified by functional ELISA and cell proliferation assay. |
| Key Features | Active isoform 6 fragment; 20-247 aa; C-terminal 6xHis tag; mammalian cell expression. |
| Purity | >=95% as determined by SDS-PAGE; >=95% as determined by SEC-HPLC. |
| Activity / Unit Definition | Functional ELISA showed binding to human ERBB3 with an EC50 of 18.24-23.66 ng/mL. The ED50 for dose-dependent stimulation of MCF-7 cell proliferation was 2.626-7.598 ng/mL. |
| Molecular Weight | 27.1 kDa |
| Source / Origin | Human protein produced in Mammalian cell. |
| pH Range / Optimal pH | pH 8.0 |
| Expiration Date / Stability | Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C. |
| Regulatory / Compliance | For research use only; not for clinical diagnosis or treatment. |
| Compatibility | Suitable for functional ELISA, ERBB3-binding studies, and MCF-7 cell proliferation assays. |
| Recommended Buffer System | 20 mM Tris-HCl, 0.5 M NaCl, 6% trehalose, pH 8.0 |
| Application Notes / Precautions | Briefly centrifuge the vial before opening. Reconstitute with sterile deionized water to 0.1-1.0 mg/mL. For long-term storage, add 5-50% glycerol as appropriate, divide into aliquots, and freeze. Avoid repeated freezing and thawing. A working aliquot may be kept at 4 C for up to one week. |
| Alternative Names / Synonyms | Pro-NRG1; GGF; HGL; HRGA; NDF; SMDF |
| Lead Time | 3-7 business days |
| Availability Status | In Stock |
| Host Species | Human |
| Tag Information | C-terminal 6xHis tag |
| Amino Acid Sequence | SGKKPESAAGSQSPALPPRLKEMKSQESAAGSKLVLRCETSSEYSSLRFKWFKNGNELNRKNKPQNIKIQKKPGKSELRINKASLADSGEYMCKVISKLGNDSASANITIVESNEIITGMPASTEGAYVSSESPIRISVSTEGANTSSSTSTSTTGTSHLVKCAEKEKTFCVNGGECFMVKDLSNPSRYLCKCPNEFTGDRCQNYVMASFYKHLGIEFMEAEELYQKR |
| UniProt Accession | Q02297 |
| Protein Length | 228 aa |
| Expression Region | 20-247 aa |
| Endotoxin Level | <1.0 EU/ug as determined by the LAL method. |
For research use only, not for clinical use.
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