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| Product Name | Recombinant Human N-Terminal Xaa-Pro-Lys N-Methyltransferase 1 (NTMT1), Active |
| Catalog No. | PTR-HMM-0153 |
| Description | An active recombinant human NTMT1 construct representing the full-length mature protein (2-223 aa) and produced in E. coli. The protein carries a N-terminal GST tag and is supplied for research use. |
| Intended Use | For research use in functional ELISA; protein-interaction studies. |
| Principle / Technology | Recombinant expression in E. coli, followed by purification and functional qualification with sDS-PAGE; functional ELISA. |
| Detection Method | SDS-PAGE; functional ELISA |
| Sample Type | Purified recombinant protein |
| Performance Range / Specifications | ≥85% by SDS-PAGE; molecular weight 52.7 kDa; package options: 20 µg; 100 µg; 1 mg. |
| Sensitivity / LOD | EC50 3.029-5.404 µg/mL for CCL5 binding. |
| Specificity | Binds human CCL5 in the reported assay. |
| Reaction Conditions / Protocol | Use the liquid material as supplied, or briefly centrifuge and reconstitute lyophilized material with sterile deionized water to 0.1-1.0 mg/mL. |
| Components / Formulation | Liquid formulation: Tris- or PBS-based buffer containing 5-50% glycerol; lyophilized formulation: Tris- or PBS-based buffer containing 6% trehalose |
| Storage Conditions | Store at -20°C or -80°C. Prepare working aliquots after opening or reconstitution and avoid repeated freeze-thaw cycles. |
| Shelf Life | Liquid material: up to 6 months at -20°C to -80°C; lyophilized material: up to 12 months at -20°C to -80°C. |
| Package Specifications | 20 µg; 100 µg; 1 mg |
| Product Form | Liquid or lyophilized |
| Quality Control | Purity assessed by SDS-PAGE; functional performance verified in the stated bioassay. |
| Key Features | Functionally tested active protein; defined 2-223 aa construct; N-terminal GST tag; ≥85% by SDS-PAGE. |
| Purity | ≥85% by SDS-PAGE |
| Activity / Unit Definition | Functional ELISA showed binding to human CCL5 with an EC50 of 3.029-5.404 µg/mL. |
| Molecular Weight | 52.7 kDa |
| Source / Origin | Human protein produced in E. coli. |
| Expiration Date / Stability | Liquid material: up to 6 months at -20°C to -80°C; lyophilized material: up to 12 months at -20°C to -80°C. |
| Regulatory / Compliance | For research use only; not for clinical diagnosis or treatment. |
| Compatibility | Functional ELISA; protein-interaction studies |
| Recommended Buffer System | Liquid formulation: Tris- or PBS-based buffer containing 5-50% glycerol; lyophilized formulation: Tris- or PBS-based buffer containing 6% trehalose |
| Application Notes / Precautions | For long-term use, store in working aliquots and avoid repeated freeze-thaw cycles. Reconstituted working material may be kept at 4°C for up to one week. |
| Alternative Names / Synonyms | Alpha N-terminal protein methyltransferase 1A; NTM1A |
| Lead Time | Contact for delivery time |
| Availability Status | In Stock |
| Host Species | Human |
| Tag Information | N-terminal GST tag |
| Amino Acid Sequence | TSEVIEDEKQFYSKAKTYWKQIPPTVDGMLGGYGHISSIDINSSRKFLQRFLREGPNKTGTSCALDCGAGIGRITKRLLLPLFREVDMVDITEDFLVQAKTYLGEEGKRVRNYFCCGLQDFTPEPDSYDVIWIQWVIGHLTDQHLAEFLRRCKGSLRPNGIIVIKDNMAQEGVILDDVDSSVCRDLDVVRRIICSAGLSLLAEERQENLPDEIYHVYSFALR |
| UniProt Accession | Q9BV86 |
| Protein Length | 222 amino acids |
| Expression Region | 2-223 aa |
For research use only, not for clinical use.
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