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| Product Name | Recombinant Human G Protein-Coupled Receptor Family C Group 5 Member D (GPRC5D) VLPs, Active |
| Catalog No. | PTR-HMM-0270 |
| Description | A full-length active human GPRC5D membrane protein displayed in a mammalian-cell-derived virus-like particle format. The C-terminally His-tagged preparation shows specific antibody binding in a functional ELISA. |
| Intended Use | Suitable for GPRC5D antibody screening, functional ELISA, GPCR target research, and VLP-based membrane-protein assays. |
| Principle / Technology | Full-length membrane protein is expressed in mammalian cells and presented in a virus-like particle format that preserves a membrane-associated antigenic conformation for binding analysis. |
| Detection Method | Functional ELISA; anti-His immunodetection under denaturing conditions; LAL assay |
| Sample Type | Purified recombinant VLP preparation |
| Performance Range / Specifications | EC50: 8.847-10.38 ng/mL. Molecular weight: 40.6 kDa. Package sizes: 20 ug; 100 ug; 1 mg. |
| Sensitivity / LOD | EC50: 8.847-10.38 ng/mL. |
| Specificity | Binding activity was demonstrated with a GPRC5D-specific recombinant antibody. |
| Reaction Conditions / Protocol | Immobilize human GPRC5D VLPs at 5 ug/mL and measure binding to a GPRC5D-specific recombinant antibody by functional ELISA. |
| Components / Formulation | PBS, 6% trehalose, pH 7.4 |
| Storage Conditions | Store at -20 C or -80 C upon receipt. Aliquot before repeated use and avoid repeated freeze-thaw cycles. |
| Shelf Life | Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C. |
| Package Specifications | 20 ug; 100 ug; 1 mg |
| Product Form | Lyophilized powder |
| Quality Control | endotoxin tested by LAL assay; biological activity validated by functional testing. |
| Key Features | Active full-length human GPRC5D VLPs; 345 aa; C-terminal 10xHis tag; mammalian expression; low endotoxin. |
| Activity / Unit Definition | In a functional ELISA, immobilized human GPRC5D at 5 ug/mL bound a GPRC5D-specific recombinant antibody with an EC50 of 8.847-10.38 ng/mL. |
| Molecular Weight | 40.6 kDa |
| Source / Origin | Human protein produced in Mammalian cells. |
| pH Range / Optimal pH | pH 7.4 |
| Expiration Date / Stability | Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C. |
| Regulatory / Compliance | For research use only; not for clinical diagnosis or treatment. |
| Compatibility | Suitable for functional ELISA, membrane receptor antibody-binding studies, and VLP-based assay development. |
| Recommended Buffer System | PBS, 6% trehalose, pH 7.4 |
| Application Notes / Precautions | Briefly centrifuge the vial before opening. Reconstitute with sterile deionized water to 0.1-1.0 mg/mL when applicable. For long-term storage, add 5-50% glycerol as appropriate, divide into aliquots, and freeze. Avoid repeated freezing and thawing. A working aliquot may be kept at 4 C for up to one week. |
| Lead Time | 3-7 business days |
| Availability Status | In Stock |
| Host Species | Human |
| Tag Information | C-terminal 10xHis tag; detectable under denaturing conditions |
| Amino Acid Sequence | MYKDCIESTGDYFLLCDAEGPWGIILESLAILGIVVTILLLLAFLFLMRKIQDCSQWNVLPTQLLFLLSVLGLFGLAFAFIIELNQQTAPVRYFLFGVLFALCFSCLLAHASNLVKLVRGCVSFSWTTILCIAIGCSLLQIIIATEYVTLIMTRGMMFVNMTPCQLNVDFVVLLVYVLFLMALTFFVSKATFCGPCENWKQHGRLIFITVLFSIIIWVVWISMLLRGNPQFQRQPQWDDPVVCIALVTNAWVFLLLYIVPELCILYRSCRQECPLQGNACPVTAYQHSFQVENQELSRARDSDGAEEDVALTSYGTPIQPQTVDPTQECFIPQAKLSPQQDAGGV |
| UniProt Accession | Q9NZD1 |
| Protein Length | 345 aa |
| Expression Region | 1-345 aa |
| Endotoxin Level | <1.0 EU/ug by the LAL method. |
For research use only, not for clinical use.
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