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| Product Name | Recombinant Human Claudin-18.2 (CLDN18.2)-VLPs, Full Length, Active |
| Catalog No. | PTR-HMM-0230 |
| Description | A full-length human CLDN18 isoform A2 membrane protein spanning residues 1-261 and presented as virus-like particles, produced in mammalian cells with a C-terminal 10xHis tag. Binding activity and particle morphology were verified experimentally. |
| Intended Use | Suitable for functional ELISA, antibody screening, CLDN18.2-targeted oncology research, immunization studies, and membrane protein analysis. |
| Principle / Technology | A defined recombinant protein region is expressed and purified, then its structural quality and biological function are evaluated using the listed biochemical binding or activity assays. |
| Detection Method | Functional ELISA; Western blot; transmission electron microscopy; LAL assay |
| Sample Type | Purified recombinant protein |
| Performance Range / Specifications | Molecular weight: 29.1 kDa. Package sizes: 20 ug; 100 ug; 1 mg. VLP-like morphology confirmed by transmission electron microscopy. |
| Sensitivity / LOD | Functional ELISA EC50: 5.225-9.256 ng/mL for binding to an anti-CLDN18.2 recombinant monoclonal antibody. |
| Specificity | Binding activity was demonstrated with an anti-CLDN18.2 recombinant monoclonal antibody. |
| Reaction Conditions / Protocol | In a functional ELISA, immobilized CLDN18.2-VLPs at 5 ug/mL bound an anti-CLDN18.2 recombinant monoclonal antibody, with an EC50 of 5.225-9.256 ng/mL. VLP-like structures were confirmed by transmission electron microscopy. |
| Components / Formulation | PBS, 6% trehalose, pH 7.4 |
| Storage Conditions | Store at -20 C or -80 C upon receipt. Aliquot before repeated use and avoid repeated freeze-thaw cycles. |
| Shelf Life | Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C. |
| Package Specifications | 20 ug; 100 ug; 1 mg |
| Product Form | Lyophilized powder |
| Quality Control | Biological activity verified by functional ELISA; oligomeric bands assessed by Western blot; VLP-like particles confirmed by transmission electron microscopy; endotoxin tested by LAL assay. |
| Key Features | Active full-length CLDN18.2-VLPs; 1-261 aa; native-like membrane presentation; C-terminal 10xHis tag. |
| Activity / Unit Definition | In a functional ELISA, immobilized CLDN18.2-VLPs at 5 ug/mL bound an anti-CLDN18.2 recombinant monoclonal antibody, with an EC50 of 5.225-9.256 ng/mL. VLP-like structures were confirmed by transmission electron microscopy. |
| Molecular Weight | 29.1 kDa |
| Source / Origin | Human protein produced in mammalian cells and assembled in a VLP format. |
| pH Range / Optimal pH | pH 7.4 |
| Shipping Conditions | Lyophilized material is normally shipped with blue ice packs; liquid format requires dry ice by prior arrangement. |
| Expiration Date / Stability | Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C. |
| Regulatory / Compliance | For research use only; not for clinical diagnosis or treatment. |
| Compatibility | Suitable for functional ELISA, Western blot, antibody screening, and CLDN18.2 immunization research. |
| Recommended Buffer System | PBS, 6% trehalose, pH 7.4 |
| Application Notes / Precautions | Briefly centrifuge the vial before opening. Reconstitute with sterile deionized water to 0.1-1.0 mg/mL. For long-term storage, add 5-50% glycerol as appropriate, divide into aliquots, and freeze. Avoid repeated freezing and thawing. A working aliquot may be kept at 4 C for up to one week. For immunization studies, optimize the antigen dose, schedule, and adjuvant for the experimental system. |
| Alternative Names / Synonyms | CLDN18; UNQ778/PRO1572; Claudin-18 |
| Lead Time | 3-7 business days |
| Availability Status | In Stock |
| Host Species | Human |
| Tag Information | C-terminal 10xHis tag; detectable under denaturing conditions |
| Amino Acid Sequence | MAVTACQGLGFVVSLIGIAGIIAATCMDQWSTQDLYNNPVTAVFNYQGLWRSCVRESSGFTECRGYFTLLGLPAMLQAVRALMIVGIVLGAIGLLVSIFALKCIRIGSMEDSAKANMTLTSGIMFIVSGLCAIAGVSVFANMLVTNFWMSTANMYTGMGGMVQTVQTRYTFGAALFVGWVAGGLTLIGGVMMCIACRGLAPEETNYKAVSYHASGHSVAYKPGGFKASTGFGSNTKNKKIYDGGARTEDEVQSYPSKHDYV |
| UniProt Accession | P56856-2 |
| Protein Length | 261 aa |
| Expression Region | 1-261 aa |
| Endotoxin Level | <1.0 EU/ug by the LAL method. |
For research use only, not for clinical use.
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