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| Product Name | Recombinant Human CD44 Antigen (CD44), Partial, Biotinylated, Active |
| Catalog No. | PTR-HMM-0203 |
| Description | A biotinylated recombinant human CD44 region spanning residues 21-220, produced in mammalian cells with a C-terminal mFc-Avi tag. Antibody-binding activity was verified by functional ELISA. |
| Intended Use | Suitable for functional ELISA, biotin-based binding assays, and CD44 target research. |
| Principle / Technology | A defined recombinant protein region is expressed and purified, then its structural quality and biological function are evaluated using the listed biochemical binding or activity assays. |
| Detection Method | SDS-PAGE; SEC-HPLC; functional ELISA; LAL assay |
| Sample Type | Purified recombinant protein |
| Performance Range / Specifications | Purity: >=90% as determined by SDS-PAGE; >=95% as determined by SEC-HPLC. Molecular weight: 51.0 kDa. Package sizes: 20 ug; 100 ug; 1 mg. |
| Sensitivity / LOD | In a functional ELISA, an immobilized anti-CD44 mouse monoclonal antibody at 2 ug/mL bound biotinylated human CD44 with an EC50 of 2.865-5.099 ng/mL. |
| Specificity | Binding activity was demonstrated with an anti-CD44 mouse monoclonal antibody. |
| Reaction Conditions / Protocol | In a functional ELISA, an immobilized anti-CD44 mouse monoclonal antibody at 2 ug/mL bound biotinylated human CD44 with an EC50 of 2.865-5.099 ng/mL. |
| Components / Formulation | PBS, 6% trehalose, pH 7.4 |
| Storage Conditions | Store at -20 C or -80 C upon receipt. Aliquot before repeated use and avoid repeated freeze-thaw cycles. |
| Shelf Life | Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C. |
| Package Specifications | 20 ug; 100 ug; 1 mg |
| Product Form | Lyophilized powder |
| Quality Control | Purity evaluated by SDS-PAGE and SEC-HPLC; endotoxin tested by LAL assay; biological activity verified by functional ELISA. |
| Key Features | Biotinylated active recombinant protein; 21-220 aa; C-terminal mFc-Avi tag; mammalian cell expression. |
| Purity | >=90% as determined by SDS-PAGE; >=95% as determined by SEC-HPLC. |
| Activity / Unit Definition | In a functional ELISA, an immobilized anti-CD44 mouse monoclonal antibody at 2 ug/mL bound biotinylated human CD44 with an EC50 of 2.865-5.099 ng/mL. |
| Molecular Weight | 51.0 kDa |
| Source / Origin | Human protein produced in Mammalian cell. |
| pH Range / Optimal pH | pH 7.4 |
| Expiration Date / Stability | Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C. |
| Regulatory / Compliance | For research use only; not for clinical diagnosis or treatment. |
| Compatibility | Suitable for functional ELISA, biotin-based binding assays, and CD44 target research. |
| Recommended Buffer System | PBS, 6% trehalose, pH 7.4 |
| Application Notes / Precautions | Briefly centrifuge the vial before opening. Reconstitute with sterile deionized water to 0.1-1.0 mg/mL. For long-term storage, add 5-50% glycerol as appropriate, divide into aliquots, and freeze. Avoid repeated freezing and thawing. A working aliquot may be kept at 4 C for up to one week. |
| Alternative Names / Synonyms | CDw44; Epican; ECMR-III; GP90; Hermes antigen; Hyaluronate receptor; PGP-1; LHR; MDU2; MDU3; MIC4 |
| Lead Time | 3-7 business days |
| Availability Status | In Stock |
| Host Species | Human |
| Tag Information | C-terminal mFc-Avi tag |
| Amino Acid Sequence | QIDLNITCRFAGVFHVEKNGRYSISRTEAADLCKAFNSTLPTMAQMEKALSIGFETCRYGFIEGHVVIPRIHPNSICAANNTGVYILTSNTSQYDTYCFNASAPPEEDCTSVTDLPNAFDGPITITIVNRDGTRYVQKGEYRTNPEDIYPSNPTDDDVSSGSSSERSSTSGGYIFYTFSTVHPIPDEDSPWITDSTDRIP |
| UniProt Accession | P16070 |
| Protein Length | 200 aa |
| Expression Region | 21-220 aa |
| Endotoxin Level | <1.0 EU/ug as determined by the LAL method. |
For research use only, not for clinical use.
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