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| Product Name | Recombinant Human CD40 Ligand (CD40LG/TNFSF5), Partial, Active |
| Catalog No. | PTR-HMM-0186 |
| Description | A mammalian-cell-expressed recombinant human CD40 ligand region spanning residues 113-261 with an N-terminal hFc-Flag tag. CD40-binding activity was confirmed by functional ELISA and LSPR. |
| Intended Use | Suitable for functional ELISA, LSPR, CD40 interaction studies, and antibody-binding assays. |
| Principle / Technology | A defined recombinant protein region is expressed and purified, then its structural quality and biological function are evaluated using the listed biochemical binding or activity assays. |
| Detection Method | SDS-PAGE; functional ELISA; LSPR; LAL assay |
| Sample Type | Purified recombinant protein |
| Performance Range / Specifications | Purity: >=90% as determined by SDS-PAGE. Molecular weight: 44.5 kDa. Package sizes: 10 ug; 20 ug; 50 ug; 100 ug; 200 ug; 500 ug; 1 mg. |
| Sensitivity / LOD | Functional ELISA showed binding to an anti-CD40LG monoclonal antibody with an EC50 of 2.353-3.232 ng/mL and binding to immobilized CD40 with an EC50 of 3.112-3.858 ng/mL. LSPR measured a CD40-CD40LG affinity constant of 2.06 nM. |
| Specificity | Binding activity was demonstrated with CD40 and an anti-CD40LG monoclonal antibody. |
| Reaction Conditions / Protocol | Functional ELISA showed binding to an anti-CD40LG monoclonal antibody with an EC50 of 2.353-3.232 ng/mL and binding to immobilized CD40 with an EC50 of 3.112-3.858 ng/mL. LSPR measured a CD40-CD40LG affinity constant of 2.06 nM. |
| Components / Formulation | PBS, 6% trehalose, pH 7.4 |
| Storage Conditions | Store at -20 C or -80 C upon receipt. Aliquot before repeated use and avoid repeated freeze-thaw cycles. |
| Shelf Life | Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C. |
| Package Specifications | 10 ug; 20 ug; 50 ug; 100 ug; 200 ug; 500 ug; 1 mg |
| Product Form | Lyophilized powder |
| Quality Control | Purity evaluated by SDS-PAGE; endotoxin tested by LAL assay; biological activity verified by functional ELISA and LSPR. |
| Key Features | Active recombinant protein; 113-261 aa; N-terminal hFc-Flag tag; Mammalian cell. |
| Purity | >=90% as determined by SDS-PAGE. |
| Activity / Unit Definition | Functional ELISA showed binding to an anti-CD40LG monoclonal antibody with an EC50 of 2.353-3.232 ng/mL and binding to immobilized CD40 with an EC50 of 3.112-3.858 ng/mL. LSPR measured a CD40-CD40LG affinity constant of 2.06 nM. |
| Molecular Weight | 44.5 kDa |
| Source / Origin | Human protein produced in Mammalian cell. |
| pH Range / Optimal pH | pH 7.4 |
| Expiration Date / Stability | Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C. |
| Regulatory / Compliance | For research use only; not for clinical diagnosis or treatment. |
| Compatibility | Suitable for functional ELISA, LSPR, CD40 interaction studies, and antibody-binding assays. |
| Recommended Buffer System | PBS, 6% trehalose, pH 7.4 |
| Application Notes / Precautions | Briefly centrifuge the vial before opening. Reconstitute with sterile deionized water to 0.1-1.0 mg/mL. For long-term storage, add 5-50% glycerol as appropriate, divide into aliquots, and freeze. Avoid repeated freezing and thawing. A working aliquot may be kept at 4 C for up to one week. |
| Alternative Names / Synonyms | CD40L; TNFSF5; TRAP |
| Lead Time | 3-7 business days |
| Availability Status | In Stock |
| Host Species | Human |
| Tag Information | N-terminal hFc-Flag tag |
| Amino Acid Sequence | MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL |
| UniProt Accession | P29965 |
| Protein Length | 149 aa |
| Expression Region | 113-261 aa |
| Endotoxin Level | <1.0 EU/ug as determined by the LAL method. |
For research use only, not for clinical use.
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