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| Product Name | Recombinant Human C5a Anaphylatoxin Chemotactic Receptor 1 (C5AR1/CD88) VLPs, Active |
| Catalog No. | PTR-HMM-0263 |
| Description | A full-length active human C5AR1 membrane protein presented in a mammalian-cell-derived virus-like particle format. The preparation carries a C-terminal 10xHis tag and shows specific antibody binding in a functional ELISA. |
| Intended Use | Suitable for C5AR1 antibody screening, functional ELISA, complement receptor research, and membrane-protein assay development. |
| Principle / Technology | Full-length membrane protein is expressed in mammalian cells and presented in a virus-like particle format that preserves a membrane-associated antigenic conformation for binding analysis. |
| Detection Method | Functional ELISA; anti-His immunodetection under denaturing conditions; transmission electron microscopy; LAL assay |
| Sample Type | Purified recombinant VLP preparation |
| Performance Range / Specifications | EC50: 1.239-3.760 ng/mL. Molecular weight: 41.1 kDa. Package sizes: 20 ug; 100 ug; 1 mg. |
| Sensitivity / LOD | EC50: 1.239-3.760 ng/mL. |
| Specificity | Binding activity was demonstrated with a C5AR1-specific recombinant antibody. |
| Reaction Conditions / Protocol | Immobilize human C5AR1 VLPs at 10 ug/mL and measure binding to a C5AR1-specific recombinant antibody by functional ELISA. |
| Components / Formulation | PBS, 6% trehalose, pH 7.4 |
| Storage Conditions | Store at -20 C or -80 C upon receipt. Aliquot before repeated use and avoid repeated freeze-thaw cycles. |
| Shelf Life | Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C. |
| Package Specifications | 20 ug; 100 ug; 1 mg |
| Product Form | Lyophilized powder |
| Quality Control | endotoxin tested by LAL assay; biological activity validated by functional testing. |
| Key Features | Active full-length human C5AR1 VLPs; 350 aa; C-terminal 10xHis tag; mammalian expression; VLP morphology confirmed by electron microscopy; low endotoxin. |
| Activity / Unit Definition | In a functional ELISA, immobilized human C5AR1 at 10 ug/mL bound a C5AR1-specific recombinant antibody with an EC50 of 1.239-3.760 ng/mL. VLP-like structures were confirmed by transmission electron microscopy. |
| Molecular Weight | 41.1 kDa |
| Source / Origin | Human protein produced in Mammalian cells. |
| pH Range / Optimal pH | pH 7.4 |
| Expiration Date / Stability | Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C. |
| Regulatory / Compliance | For research use only; not for clinical diagnosis or treatment. |
| Compatibility | Suitable for functional ELISA, membrane receptor antibody-binding studies, and VLP-based assay development. |
| Recommended Buffer System | PBS, 6% trehalose, pH 7.4 |
| Application Notes / Precautions | Briefly centrifuge the vial before opening. Reconstitute with sterile deionized water to 0.1-1.0 mg/mL when applicable. For long-term storage, add 5-50% glycerol as appropriate, divide into aliquots, and freeze. Avoid repeated freezing and thawing. A working aliquot may be kept at 4 C for up to one week. |
| Alternative Names / Synonyms | C5a anaphylatoxin chemotactic receptor 1; C5a receptor; C5a-R; C5aR; CD88 |
| Lead Time | 3-7 business days |
| Availability Status | In Stock |
| Host Species | Human |
| Tag Information | C-terminal 10xHis tag; detectable under denaturing conditions |
| Amino Acid Sequence | MDSFNYTTPDYGHYDDKDTLDLNTPVDKTSNTLRVPDILALVIFAVVFLVGVLGNALVVWVTAFEAKRTINAIWFLNLAVADFLSCLALPILFTSIVQHHHWPFGGAACSILPSLILLNMYASILLLATISADRFLLVFKPIWCQNFRGAGLAWIACAVAWGLALLLTIPSFLYRVVREEYFPPKVLCGVDYSHDKRRERAVAIVRLVLGFLWPLLTLTICYTFILLRTWSRRATRSTKTLKVVVAVVASFFIFWLPYQVTGIMMSFLEPSSPTFLLLKKLDSLCVSFAYINCCINPIIYVVAGQGFQGRLRKSLPSLLRNVLTEESVVRESKSFTRSTVDTMAQKTQAV |
| UniProt Accession | P21730 |
| Protein Length | 350 aa |
| Expression Region | 1-350 aa |
| Endotoxin Level | <1.0 EU/ug by the LAL method. |
For research use only, not for clinical use.
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