- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Recombinant Human B-Lymphocyte Antigen CD20 (MS4A1), Full Length, VLP, Active |
| Catalog No. | PTR-HMM-0245 |
| Description | A full-length recombinant human CD20/MS4A1 membrane protein produced in mammalian cells and presented in a virus-like particle format. VLP-like structures were confirmed by TEM, protein identity was supported by LC-MS/MS, and antibody-binding activity was verified by functional ELISA. |
| Intended Use | Suitable for antibody-binding studies, B-cell marker research, cancer research, immunogenicity studies, and assay development. |
| Principle / Technology | A defined recombinant protein region is expressed and purified, then its structural quality and biological function are evaluated using the listed biochemical binding or activity assays. |
| Detection Method | SEC-HPLC; functional ELISA; TEM; LC-MS/MS; Western blot; LAL assay |
| Sample Type | Purified recombinant protein |
| Performance Range / Specifications | Purity: >=90% by SEC-HPLC.. Molecular weight: 34.4 kDa. Package sizes: 20 ug; 100 ug; 1 mg. |
| Sensitivity / LOD | In a functional ELISA, immobilized human CD20 at 2 ug/mL bound a CD20-specific recombinant antibody with an EC50 of 3.243-7.085 ng/mL. |
| Specificity | Binding activity was demonstrated with a CD20-specific recombinant antibody; full-length protein identity was supported by LC-MS/MS. |
| Reaction Conditions / Protocol | In a functional ELISA, immobilized human CD20 at 2 ug/mL bound a CD20-specific recombinant antibody with an EC50 of 3.243-7.085 ng/mL. |
| Components / Formulation | PBS, 6% trehalose, pH 7.4 |
| Storage Conditions | Store at -20 C or -80 C upon receipt. Aliquot before repeated use and avoid repeated freeze-thaw cycles. |
| Shelf Life | Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C. |
| Package Specifications | 20 ug; 100 ug; 1 mg |
| Product Form | Lyophilized powder |
| Quality Control | Purity evaluated by sec-hplc; endotoxin tested by lal assay; biological activity verified by functional elisa; particle morphology confirmed by tem; protein identity supported by lc-ms/ms. |
| Key Features | Active full-length human CD20; VLP presentation; C-terminal 10xHis tag; mammalian cell expression; particle structure confirmed by TEM. |
| Purity | >=90% by SEC-HPLC. |
| Activity / Unit Definition | In a functional ELISA, immobilized human CD20 at 2 ug/mL bound a CD20-specific recombinant antibody with an EC50 of 3.243-7.085 ng/mL. |
| Molecular Weight | 34.4 kDa |
| Source / Origin | Human protein produced in Mammalian cells. |
| pH Range / Optimal pH | pH 7.4 |
| Expiration Date / Stability | Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C. |
| Regulatory / Compliance | For research use only; not for clinical diagnosis or treatment. |
| Compatibility | Suitable for antibody-binding studies, B-cell marker research, cancer research, immunogenicity studies, and assay development. |
| Recommended Buffer System | PBS, 6% trehalose, pH 7.4 |
| Application Notes / Precautions | Briefly centrifuge the vial before opening. Reconstitute with sterile deionized water to 0.1-1.0 mg/mL when applicable. For long-term storage, add 5-50% glycerol as appropriate, divide into aliquots, and freeze. Avoid repeated freezing and thawing. A working aliquot may be kept at 4 C for up to one week. |
| Alternative Names / Synonyms | MS4A1; CD20; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; CD antigen CD20 |
| Lead Time | 3-7 business days |
| Availability Status | In Stock |
| Host Species | Human |
| Tag Information | C-terminal 10xHis tag, detectable under denaturing conditions |
| Amino Acid Sequence | MTTPRNSVNGTFPAEPMKGPIAMQSGPKPLFRRMSSLVGPTQSFFMRESKTLGAVQIMNGLFHIALGGLLMIPAGIYAPICVTVWYPLWGGIMYIISGSLLAATEKNSRKCLVKGKMIMNSLSLFAAISGMILSIMDILNIKISHFLKMESLNFIRAHTPYINIYNCEPANPSEKNSPSTQYCYSIQSLFLGILSVMLIFAFFQELVIAGIVENEWKRTCSRPKSNIVLLSAEEKKEQTIEIKEEVVGLTETSSQPKNEEDIEIIPIQEEEEEETETNFPEPPQDQESSPIENDSSP |
| UniProt Accession | P11836 |
| Protein Length | 297 aa |
| Expression Region | 1-297 aa |
| Endotoxin Level | <1.0 EU/ug by the LAL method. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
