Recombinant Human Angiotensin-Converting Enzyme 2 (ACE2), Partial, Active

Name: Recombinant Human Angiotensin-Converting Enzyme 2 (ACE2), Partial, Active
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Cat.No: PTR-HMM-0143 Datasheet

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Product Name	Recombinant Human Angiotensin-Converting Enzyme 2 (ACE2), Partial, Active
Catalog No.	PTR-HMM-0143
Description	An active recombinant human ACE2 construct representing the partial extracellular region (18-740 aa) and produced in mammalian cells. The protein carries a C-terminal hFc1 tag and is supplied for research use.
Intended Use	For research use in functional ELISA; LSPR; viral receptor-binding studies.
Principle / Technology	Recombinant expression in mammalian cells, followed by purification and functional qualification with sDS-PAGE; functional ELISA; LSPR.
Detection Method	SDS-PAGE; functional ELISA; LSPR
Sample Type	Purified recombinant protein
Performance Range / Specifications	≥90% by SDS-PAGE; molecular weight 112.5 kDa; package options: 10 µg; 20 µg; 50 µg; 100 µg; 200 µg; 500 µg; 1 mg.
Sensitivity / LOD	ELISA EC50 2.785-103.6 ng/mL; LSPR KD 13.8 nM or 100 nM, depending on the spike/RBD construct.
Specificity	Binds SARS-CoV-2 spike receptor-binding-domain constructs.
Reaction Conditions / Protocol	Briefly centrifuge the vial before opening, then reconstitute with sterile deionized water to 0.1-1.0 mg/mL.
Components / Formulation	PBS containing 6% trehalose, pH 7.4
Storage Conditions	Store at -20°C or -80°C. Prepare working aliquots after opening or reconstitution and avoid repeated freeze-thaw cycles.
Shelf Life	Lyophilized material: up to 12 months at -20°C to -80°C.
Package Specifications	10 µg; 20 µg; 50 µg; 100 µg; 200 µg; 500 µg; 1 mg
Product Form	Lyophilized powder
Quality Control	Purity assessed by SDS-PAGE; functional performance verified in the stated bioassay; endotoxin level <1.0 EU/µg.
Key Features	Functionally tested active protein; defined 18-740 aa construct; C-terminal hFc1 tag; ≥90% by SDS-PAGE.
Purity	≥90% by SDS-PAGE
Activity / Unit Definition	Functional ELISA demonstrated binding to SARS-CoV-2 spike and receptor-binding-domain constructs with EC50 values ranging from 2.785 to 103.6 ng/mL. LSPR measurements gave KD values of 13.8 nM and 100 nM for different RBD constructs.
Molecular Weight	112.5 kDa
Source / Origin	Human protein produced in mammalian cells.
pH Range / Optimal pH	pH 7.4
Expiration Date / Stability	Lyophilized material: up to 12 months at -20°C to -80°C.
Regulatory / Compliance	For research use only; not for clinical diagnosis or treatment.
Compatibility	Functional ELISA; LSPR; viral receptor-binding studies
Recommended Buffer System	PBS containing 6% trehalose, pH 7.4
Application Notes / Precautions	After reconstitution, keep a working aliquot at 4°C for no longer than one week. For longer storage, prepare aliquots and avoid repeated freeze-thaw cycles.
Alternative Names / Synonyms	ACEH; angiotensin-converting enzyme homolog; ACE-related carboxypeptidase
Lead Time	3-7 business days
Availability Status	In Stock
Host Species	Human
Tag Information	C-terminal hFc1 tag
Amino Acid Sequence	QSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVS
UniProt Accession	Q9BYF1
Protein Length	723 amino acids
Expression Region	18-740 aa
Endotoxin Level	<1.0 EU/µg

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For research use only, not for clinical use.