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| Product Name | Recombinant Human 5'-Nucleotidase (NT5E/CD73), Mature Protein, Active |
| Catalog No. | PTR-HMM-0202 |
| Description | A recombinant human mature NT5E/CD73 ectodomain spanning residues 27-549, produced in mammalian cells with a C-terminal 6xHis tag. Antibody-binding activity was confirmed by functional ELISA. |
| Intended Use | Suitable for functional ELISA, antibody-binding studies, and NT5E/CD73 target research. |
| Principle / Technology | A defined recombinant protein region is expressed and purified, then its structural quality and biological function are evaluated using the listed biochemical binding or activity assays. |
| Detection Method | SDS-PAGE; functional ELISA; LAL assay |
| Sample Type | Purified recombinant protein |
| Performance Range / Specifications | Purity: >=95% as determined by SDS-PAGE. Molecular weight: 60.2 kDa. Package sizes: 20 ug; 100 ug; 1 mg. |
| Sensitivity / LOD | In a functional ELISA, immobilized CD73 at 2 ug/mL bound an anti-CD73 rabbit monoclonal antibody with an EC50 of 3.212-4.525 ng/mL. |
| Specificity | Antibody-binding activity was demonstrated with an anti-CD73 rabbit monoclonal antibody. |
| Reaction Conditions / Protocol | In a functional ELISA, immobilized CD73 at 2 ug/mL bound an anti-CD73 rabbit monoclonal antibody with an EC50 of 3.212-4.525 ng/mL. |
| Components / Formulation | 20 mM Tris-HCl, 0.5 M NaCl, 6% trehalose, pH 8.0 |
| Storage Conditions | Store at -20 C or -80 C upon receipt. Aliquot before repeated use and avoid repeated freeze-thaw cycles. |
| Shelf Life | Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C. |
| Package Specifications | 20 ug; 100 ug; 1 mg |
| Product Form | Lyophilized powder |
| Quality Control | Purity evaluated by SDS-PAGE; endotoxin tested by LAL assay; biological activity verified by functional ELISA. |
| Key Features | Active mature recombinant protein; 27-549 aa; C-terminal 6xHis tag; mammalian cell expression. |
| Purity | >=95% as determined by SDS-PAGE. |
| Activity / Unit Definition | In a functional ELISA, immobilized CD73 at 2 ug/mL bound an anti-CD73 rabbit monoclonal antibody with an EC50 of 3.212-4.525 ng/mL. |
| Molecular Weight | 60.2 kDa |
| Source / Origin | Human protein produced in Mammalian cell. |
| pH Range / Optimal pH | pH 8.0 |
| Expiration Date / Stability | Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C. |
| Regulatory / Compliance | For research use only; not for clinical diagnosis or treatment. |
| Compatibility | Suitable for functional ELISA, antibody-binding studies, and NT5E/CD73 target research. |
| Recommended Buffer System | 20 mM Tris-HCl, 0.5 M NaCl, 6% trehalose, pH 8.0 |
| Application Notes / Precautions | Briefly centrifuge the vial before opening. Reconstitute with sterile deionized water to 0.1-1.0 mg/mL. For long-term storage, add 5-50% glycerol as appropriate, divide into aliquots, and freeze. Avoid repeated freezing and thawing. A working aliquot may be kept at 4 C for up to one week. |
| Alternative Names / Synonyms | 5'-NT; Ecto-5'-nucleotidase; CD73; NT5; NTE |
| Lead Time | 3-7 business days |
| Availability Status | In Stock |
| Host Species | Human |
| Tag Information | C-terminal 6xHis tag |
| Amino Acid Sequence | WELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHTDEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIKFS |
| UniProt Accession | P21589 |
| Protein Length | 523 aa |
| Expression Region | 27-549 aa |
| Endotoxin Level | <1.0 EU/ug as determined by the LAL method. |
For research use only, not for clinical use.
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