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| Product Name | Recombinant Escherichia coli Uncharacterized Protein YcbX (ycbX), Active |
| Catalog No. | PTR-HMM-0147 |
| Description | An active recombinant E. coli K-12 ycbX construct representing the full-length protein (1-369 aa) and produced in E. coli. The protein carries a N-terminal GST tag and is supplied for research use. |
| Intended Use | For research use in functional ELISA; bacterial protein-interaction studies. |
| Principle / Technology | Recombinant expression in E. coli, followed by purification and functional qualification with sDS-PAGE; functional ELISA. |
| Detection Method | SDS-PAGE; functional ELISA |
| Sample Type | Purified recombinant protein |
| Performance Range / Specifications | ≥85% by SDS-PAGE; molecular weight 67.6 kDa; package options: 10 µg; 20 µg; 50 µg; 100 µg; 200 µg; 500 µg; 1 mg. |
| Sensitivity / LOD | EC50 359.062-628.116 µg/mL for LdcC binding. |
| Specificity | Binds Escherichia coli LdcC in the reported assay. |
| Reaction Conditions / Protocol | Use the liquid material as supplied, or briefly centrifuge and reconstitute lyophilized material with sterile deionized water to 0.1-1.0 mg/mL. |
| Components / Formulation | Tris-based buffer containing 50% glycerol |
| Storage Conditions | Store at -20°C or -80°C. Prepare working aliquots after opening or reconstitution and avoid repeated freeze-thaw cycles. |
| Shelf Life | Liquid material: up to 6 months at -20°C to -80°C; lyophilized material: up to 12 months at -20°C to -80°C. |
| Package Specifications | 10 µg; 20 µg; 50 µg; 100 µg; 200 µg; 500 µg; 1 mg |
| Product Form | Liquid or lyophilized |
| Quality Control | Purity assessed by SDS-PAGE; functional performance verified in the stated bioassay. |
| Key Features | Functionally tested active protein; defined 1-369 aa construct; N-terminal GST tag; ≥85% by SDS-PAGE. |
| Purity | ≥85% by SDS-PAGE |
| Activity / Unit Definition | Functional ELISA demonstrated interaction with Escherichia coli LdcC with an EC50 of 359.062-628.116 µg/mL. |
| Molecular Weight | 67.6 kDa |
| Source / Origin | E. coli K-12 protein produced in E. coli. |
| Expiration Date / Stability | Liquid material: up to 6 months at -20°C to -80°C; lyophilized material: up to 12 months at -20°C to -80°C. |
| Regulatory / Compliance | For research use only; not for clinical diagnosis or treatment. |
| Compatibility | Functional ELISA; bacterial protein-interaction studies |
| Recommended Buffer System | Tris-based buffer containing 50% glycerol |
| Application Notes / Precautions | For long-term use, store in working aliquots and avoid repeated freeze-thaw cycles. Reconstituted working material may be kept at 4°C for up to one week. |
| Genotype | K-12 |
| Alternative Names / Synonyms | ycbX; b0947; JW5126 |
| Lead Time | 3-7 business days |
| Availability Status | In Stock |
| Host Species | Escherichia coli K-12 |
| Tag Information | N-terminal GST tag |
| Amino Acid Sequence | MATLIRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGTFITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIAPDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQRCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQKHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAAADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQLLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR |
| UniProt Accession | P75863 |
| Protein Length | 369 amino acids |
| Expression Region | 1-369 aa |
For research use only, not for clinical use.
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