- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Recombinant DT3C Protein for Antibody Internalization Assays, Active |
| Catalog No. | PTR-HMM-0279 |
| Description | A recombinant linked heterodimer combining diphtheria toxin-derived and protein G 3C-associated domains for antibody internalization studies. The protein is produced in E. coli with an N-terminal 6xHis tag and supports antibody-dependent cytotoxicity readouts in receptor-expressing cells. |
| Intended Use | Suitable for antibody internalization screening, receptor-mediated uptake studies, and cell-based cytotoxicity assay development. |
| Principle / Technology | DT3C forms a complex with an internalizing antibody. Following receptor-mediated uptake, the toxin-derived component produces a cell-viability response that reflects antibody internalization efficiency. |
| Detection Method | SDS-PAGE; SEC-HPLC; cell-based cytotoxicity assay; LAL assay |
| Sample Type | Purified recombinant linked heterodimer |
| Performance Range / Specifications | Purity: >=90% by SDS-PAGE and >=90% by SEC-HPLC. ED50: 0.3708-0.5608 ug/mL in CHO-K1/human CCR8 cells; 0.4661-0.8614 ug/mL in CT26/human CCR8 cells. Molecular weight: 69.4 kDa. Package sizes: 20 ug; 100 ug; 1 mg. |
| Sensitivity / LOD | ED50: 0.3708-0.5608 ug/mL in CHO-K1/human CCR8 cells; 0.4661-0.8614 ug/mL in CT26/human CCR8 cells. |
| Specificity | Activity was demonstrated in two human CCR8-expressing cell models using internalizing antibody complexes. |
| Reaction Conditions / Protocol | Prepare complexes using DT3C at 10 ug/mL and graded concentrations of the test antibody, expose receptor-expressing cells, and quantify the concentration-dependent cytotoxicity response. |
| Components / Formulation | PBS, 6% trehalose, pH 7.4 |
| Storage Conditions | Store at -20 C or -80 C upon receipt. Aliquot before repeated use and avoid repeated freeze-thaw cycles. |
| Shelf Life | Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C. |
| Package Specifications | 20 ug; 100 ug; 1 mg |
| Product Form | Lyophilized powder |
| Quality Control | Purity evaluated by SDS-PAGE and SEC-HPLC; endotoxin tested by LAL assay; activity validated in cell-based cytotoxicity assays. |
| Key Features | Linked heterodimer; N-terminal 6xHis tag; E. coli expression; dual-method purity assessment; activity verified in two CCR8-expressing cell models; low endotoxin. |
| Purity | >=90% by SDS-PAGE and >=90% by SEC-HPLC. |
| Activity / Unit Definition | At 10 ug/mL, antibody-DT3C complexes produced cytotoxic responses in CHO-K1 cells expressing human CCR8 with an ED50 of 0.3708-0.5608 ug/mL and in CT26 cells expressing human CCR8 with an ED50 of 0.4661-0.8614 ug/mL. |
| Molecular Weight | 69.4 kDa |
| Source / Origin | Recombinant DT3C linked heterodimer produced in E. coli. |
| pH Range / Optimal pH | pH 7.4 |
| Expiration Date / Stability | Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C. |
| Regulatory / Compliance | For research use only; not for clinical diagnosis or treatment. |
| Compatibility | Suitable for antibody internalization assays using receptor-expressing mammalian cell lines. |
| Recommended Buffer System | PBS, 6% trehalose, pH 7.4 |
| Application Notes / Precautions | Briefly centrifuge the vial before opening. Reconstitute with sterile deionized water to 0.1-1.0 mg/mL when applicable. For long-term storage, add 5-50% glycerol as appropriate, divide into aliquots, and freeze. Avoid repeated freezing and thawing. A working aliquot may be kept at 4 C for up to one week. |
| Alternative Names / Synonyms | Diphtheria toxin and protein G 3C domain fusion |
| Lead Time | 3-7 business days |
| Availability Status | In Stock |
| Host Species | Not applicable |
| Tag Information | N-terminal 6xHis tag |
| UniProt Accession | P00588; P19909 |
| Protein Length | Linked heterodimer |
| Endotoxin Level | <1.0 EU/ug by the LAL method. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
