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| Product Name | Recombinant Human Programmed Cell Death Protein 1 (PDCD1/PD-1), Partial, Active |
| Catalog No. | PTR-HMM-0176 |
| Description | Human PD-1 extracellular region covering residues 25-167, produced in mammalian cells with a C-terminal 6xHis tag. The active protein is validated for binding to recombinant anti-PD-1 antibodies and nivolumab. |
| Intended Use | PD-1 antibody-binding assays, functional ELISA, immune checkpoint research, and assay development. |
| Principle / Technology | A defined recombinant protein region is expressed and purified, then its structural integrity and target-binding function are evaluated using the listed biochemical binding assays. |
| Detection Method | SDS-PAGE; SEC-HPLC; functional ELISA |
| Sample Type | Purified recombinant protein |
| Performance Range / Specifications | >=95% by SDS-PAGE; >=95% by SEC-HPLC; calculated molecular weight 18.2 kDa; package options: 20 ug; 100 ug; 1 mg. |
| Sensitivity / LOD | In functional ELISA, immobilized PD-1 bound a recombinant anti-PD-1 antibody with an EC50 of 6.087-7.854 ng/mL and nivolumab with an EC50 of 9.713-12.39 ng/mL. |
| Specificity | Binds anti-PD-1 antibodies, including nivolumab. |
| Reaction Conditions / Protocol | Briefly centrifuge the vial before opening. Reconstitute with sterile deionized water to 0.1-1.0 mg/mL. For long-term storage, add 5-50% glycerol as appropriate, divide into aliquots, and freeze. |
| Components / Formulation | Tris- or PBS-based buffer containing 6% trehalose before lyophilization |
| Storage Conditions | Store at -20 C or -80 C upon receipt. Aliquot before repeated use and avoid repeated freeze-thaw cycles. |
| Shelf Life | Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C. |
| Package Specifications | 20 ug; 100 ug; 1 mg |
| Product Form | Lyophilized powder |
| Quality Control | Purity evaluated by SDS-PAGE and SEC-HPLC; endotoxin tested by LAL assay; biological activity verified by functional ELISA. |
| Key Features | Active Human recombinant protein; 25-167 aa expression region; Mammalian cell expression; C-terminal 6xHis tag; >=95% by SDS-PAGE; >=95% by SEC-HPLC; low endotoxin. |
| Purity | >=95% by SDS-PAGE; >=95% by SEC-HPLC |
| Activity / Unit Definition | In functional ELISA, immobilized PD-1 bound a recombinant anti-PD-1 antibody with an EC50 of 6.087-7.854 ng/mL and nivolumab with an EC50 of 9.713-12.39 ng/mL. |
| Molecular Weight | 18.2 kDa |
| Source / Origin | Human protein produced in Mammalian cell. |
| Expiration Date / Stability | Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C. |
| Regulatory / Compliance | For research use only; not for clinical diagnosis or treatment. |
| Compatibility | PD-1 antibody-binding assays, functional ELISA, immune checkpoint research, and assay development. |
| Recommended Buffer System | Tris- or PBS-based buffer containing 6% trehalose before lyophilization |
| Application Notes / Precautions | Avoid repeated freezing and thawing. A working aliquot may be kept at 4 C for up to one week. |
| Alternative Names / Synonyms | Protein PD-1; hPD-1; CD279 |
| Lead Time | 3-7 business days |
| Availability Status | In Stock |
| Host Species | Human |
| Tag Information | C-terminal 6xHis tag |
| Amino Acid Sequence | LDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQ |
| UniProt Accession | Q15116 |
| Protein Length | 143 amino acids |
| Expression Region | 25-167 aa |
| Endotoxin Level | <1.0 EU/ug by LAL assay |
For research use only, not for clinical use.
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