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| Product Name | Protein A/G Magnetic Beads for Immunoprecipitation |
| Catalog No. | PTR-HMM-0064 |
| Description | Magnetic bead-conjugated recombinant Protein A/G for the efficient capture and purification of antibodies and antibody-antigen complexes in immunoprecipitation (IP) and co-immunoprecipitation (Co-IP) applications. |
| Intended Use | Immunoprecipitation of target proteins from cell lysates and biological fluids using specific antibodies, as well as antibody purification from serum and culture supernatants. |
| Principle / Technology | Recombinant Protein A/G fusion protein covalently coupled to superparamagnetic iron oxide beads binds to the Fc region of IgG antibodies from multiple species and subclasses with high affinity. |
| Detection Method | Magnetic separation of bead-bound antibody-antigen complexes; subsequent analysis by SDS-PAGE, Western blot, or mass spectrometry |
| Sample Type | Cell lysates, tissue extracts, serum, plasma, cell culture supernatants; compatible with antibodies from human, mouse, rabbit, goat, sheep, and rat |
| Performance Range / Specifications | Binding capacity: ≥30 μg human IgG per 50 μL of settled bead slurry; typical immunoprecipitation efficiency >85% for target antigens |
| Sensitivity / LOD | Capable of enriching proteins present at <0.001% of total cellular protein with proper antibody selection |
| Specificity | Protein A/G fusion provides broad IgG subclass binding: human IgG1-4, mouse IgG1, IgG2a, IgG2b, IgG3, rabbit all subclasses, goat, sheep, bovine, and rat IgG |
| Reaction Conditions / Protocol | Incubate magnetic beads with antibody and protein sample for 30–60 minutes at room temperature or 4°C with gentle rotation, magnetically separate, wash 3–5 times, elute bound proteins |
| Components / Formulation | 50% slurry of Protein A/G-conjugated magnetic beads in PBS with 0.02% sodium azide preservative; beads are approximately 1 μm diameter with iron oxide core and hydrophilic surface coating |
| Storage Conditions | 2–8°C; do not freeze as this may damage the magnetic properties and bead integrity |
| Shelf Life | 24 months from date of manufacture |
| Package Specifications | 1 mL of 50% bead slurry (sufficient for 20–40 immunoprecipitation reactions) |
| Product Form | Dark brown magnetic bead suspension |
| Quality Control | Each lot tested for IgG binding capacity using standardized human IgG solution; non-specific binding assessed with BSA control; magnetic separation efficiency verified (>95% bead recovery within 1 minute) |
| Key Features | No centrifugation required — magnetic separation simplifies handling; Protein A/G fusion provides broader species reactivity than Protein A or G alone; low non-specific binding reduces background in Co-IP experiments |
For research use only, not for clinical use.
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