Recombinant Cynomolgus Monkey Trophoblast Glycoprotein (TPBG), Partial, Active
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Recombinant Cynomolgus Monkey Trophoblast Glycoprotein (TPBG), Partial, Active

Cat.No: PTR-HMM-0138 Datasheet

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Product Name Recombinant Cynomolgus Monkey Trophoblast Glycoprotein (TPBG), Partial, Active
Catalog No. PTR-HMM-0138
Description An active recombinant cynomolgus monkey TPBG protein representing partial, produced using Mammalian cells and purified for functional protein research.
Intended Use For protein interaction studies, antibody evaluation, assay development, functional characterization and related life-science research.
Principle / Technology Recombinant expression using Mammalian cells, followed by purification and functional qualification.
Detection Method ELISA binding assay
Sample Type Purified recombinant protein
Performance Range / Specifications Package options: 10 ug; 20 ug; 50 ug; 100 ug; 200 ug; 500 ug; 1 mg. Purity: >=95% by SDS-PAGE. Functional performance: Immobilized cynomolgus monkey TPBG binds an anti-TPBG antibody with an EC50 of 1.672-2.181 ng/mL.
Sensitivity / LOD EC50: 1.672-2.181 ng/mL
Specificity Cynomolgus monkey TPBG
Reaction Conditions / Protocol Immobilized cynomolgus monkey TPBG binds an anti-TPBG antibody with an EC50 of 1.672-2.181 ng/mL.
Components / Formulation 20 mM Tris-HCl, 0.5 M NaCl and 6% trehalose, pH 8.0
Storage Conditions Store at -20 C or -80 C. Prepare working aliquots for repeated use and avoid repeated freeze-thaw cycles.
Shelf Life Liquid: 6 months at -20 C or -80 C. Lyophilized: 12 months at -20 C or -80 C.
Package Specifications 10 ug; 20 ug; 50 ug; 100 ug; 200 ug; 500 ug; 1 mg
Product Form Lyophilized powder
Quality Control Purity is assessed by the method stated in the specification. Functional performance is qualified by elisa binding assay.
Key Features Active recombinant cynomolgus monkey TPBG; Partial; Mammalian cells; >=95% by SDS-PAGE purity; documented functional performance; endotoxin <1.0 EU/ug by LAL assay.
Purity >=95% by SDS-PAGE
Activity / Unit Definition Immobilized cynomolgus monkey TPBG binds an anti-TPBG antibody with an EC50 of 1.672-2.181 ng/mL.
Molecular Weight 39.9 kDa
Source / Origin Cynomolgus monkey; recombinant production using Mammalian cells.
pH Range / Optimal pH 8.0
Expiration Date / Stability Liquid material is stable for 6 months at -20 C or -80 C; lyophilized material is stable for 12 months at -20 C or -80 C.
Regulatory / Compliance For research use only; not for clinical diagnosis or treatment.
Compatibility ELISA; antibody binding studies; comparative oncology research
Recommended Buffer System 20 mM Tris-HCl, 0.5 M NaCl and 6% trehalose, pH 8.0
Application Notes / Precautions Briefly centrifuge lyophilized material before opening. Reconstitute with sterile deionized water to 0.1-1.0 mg/mL. For extended storage after reconstitution, add glycerol to a final concentration of 5%-50%, prepare working aliquots and avoid repeated freeze-thaw cycles. Working aliquots may be kept at 4 C for up to one week.
Alternative Names / Synonyms Trophoblast glycoprotein; TPBG; Wnt-activated inhibitory factor 1; WAIF1
Lead Time 3-7 working days
Availability Status In Stock
Host Species Cynomolgus monkey
Tag Information N-terminal 10xHis-tagged and C-terminal Myc-tagged
Amino Acid Sequence TSSASSSSSSAPFLASAASAQPPLPDQCPALCECSEAARTVKCVNRNLTEVPTDLPLYVRNLFLTGNQLAVLPAGAFARRPPLAELAALNLSGSRLDEVRGGAFEHLPSLRQLDLSHNPLAYLSPFAFSGSNASISAPSPLVELILNHIVPPDDKRQNRSFEGMVAAALVAGRALQGLHLLELASNHFLYLPRDVLAQLPSLRYLDLSNNSLVSLTYVSFRNLTHLESLHLEDNALKVLHNGTLAELQGLPHVRVFLDNNPWVCDCHMADMVTWLKQTGVVQGKDRLTCAFPEKMRNRVLLELNSADLDCDPILPPSLQTS
UniProt Accession Q4R8Y9
Protein Length 321 aa
Expression Region 35-355 aa
Endotoxin Level <1.0 EU/ug by LAL assay

For research use only, not for clinical use.

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