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| Product Name | Ready-to-Use Green Nucleic Acid Stain for Live Cells, 5 mM (Ex/Em 485/498 nm) |
| Catalog No. | RERE-0010 |
| Description | This green fluorescent dye is supplied ready to use - no DMSO dissolution or concentration adjustment - and is designed to visualise nucleic acids in both prokaryotic and eukaryotic cells. It passes through intact membranes of living cells, and once intercalated into DNA and RNA its signal jumps more than forty-fold, from a quantum yield below 0.01 in the free state to above 0.4 when bound. Fluorescence tracks nucleic acid content linearly on the FITC channel, with a 15-20 minute incubation and no fixation, permeabilisation or washing. It pairs cleanly with membrane-impermeant dyes such as propidium iodide, so live and dead cells or bacteria can be told apart without signal cross-talk. |
| Intended Use | Nucleic acid visualisation across microbiology (Gram-positive/Gram-negative viability, fungal activity, water and food contamination screening, biofilm quantification), cell biology (live-cell imaging, proliferation and apoptosis monitoring, stem cell differentiation tracking, drug metabolism effects) and industrial quality control (fermentation monitoring, microbial limits, pathogen screening). |
| Principle / Technology | The dye penetrates intact live-cell membranes and intercalates into DNA and RNA; binding lifts the quantum yield from under 0.01 to above 0.4, so fluorescence intensity reports nucleic acid content and biological activity directly. |
| Detection Method | Green fluorescence on the FITC channel, Ex 485 nm / Em 498 nm; compatible with flow cytometers, fluorescence microscopes and plate readers. |
| Sample Type | Prokaryotic cells (bacteria), eukaryotic cells (fungi, mammalian cells). |
| Performance Range / Specifications | Supplied at 5 mM, ready to use; fluorescence enhancement above 40-fold on binding; molar absorptivity above 60,000 cm-1 M-1; quantum yield below 0.01 unbound and above 0.4 bound; Ex/Em 485/498 nm; FITC channel; incubation 15-20 minutes; linear response with nucleic acid content; PI-compatible without cross-talk; low cytotoxicity at working concentrations. |
| Sensitivity / LOD | sensitivity follows the instrument and sample format. |
| Specificity | Binds DNA and RNA; membrane-permeant so it stains live cells; pairs with membrane-impermeant counterstains for viability discrimination. |
| Reaction Conditions / Protocol | Add the ready-to-use dye to the sample, incubate 15-20 minutes, then read on the FITC channel - no fixation, permeabilisation or wash steps required. |
| Components / Formulation | One vial of 100 μL dye solution at 5 mM. |
| Storage Conditions | Store at -25 to -15 °C, protected from light. |
| Shelf Life | 12 months under the recommended storage conditions. |
| Package Specifications | 100 μL per vial |
| Product Form | Ready-to-use aqueous solution at 5 mM. |
| Quality Control | Performance is anchored by the documented enhancement factor, quantum yield change and linear dose response. |
| Key Features | - Ready to use - no DMSO dissolution or calibration - More than 40-fold brightening on DNA/RNA binding - No fixation, permeabilization or wash steps - Pairs with membrane-impermeant dyes for live/dead calls - Linear response on the FITC channel across platforms |
| Concentration | 5 mM ready-to-use solution. |
| Activity / Unit Definition | No unit definition; staining performance is defined by the enhancement and linearity figures. |
| Source / Origin | Synthetic nucleic-acid-binding fluorophore, supplied as an aqueous solution. |
| pH Range / Optimal pH | Does not apply; pH is not a meaningful specification for this product type. |
| Expiration Date / Stability | Remains stable for the stated period provided the cold chain is not broken and light exposure is avoided. |
| Regulatory / Compliance | Restricted to research applications. |
| Compatibility | Works on common fluorescence platforms; match the filter set to the excitation/emission values listed for this product. |
| Recommended Buffer System | Supplied ready to use - no DMSO dissolution or concentration calibration needed. |
| Application Notes / Precautions | Protect from light throughout, keep the 15-20 minute incubation consistent, and when pairing with membrane-impermeant counterstains, titrate both dyes so the spectra stay separate. |
| Batch-to-Batch Consistency | Consistency between lots is maintained by fixed production controls and release testing against specification. |
| Model Type | Membrane-permeant nucleic acid staining dye |
| Starting Material / Tissue Site | Synthesised green fluorophore supplied as a 5 mM ready-to-use solution |
| Delivery Standard | 100 μL vial at 5 mM |
| Turnaround Time | dispatch timing is settled at purchase. |
| Readouts / Downstream Assays | Green fluorescence at Ex 485/Em 498 nm, linear with nucleic acid content. |
For research use only, not for clinical use.
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