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| Product Name | Qubit RNA High-Sensitivity Quantification Kit |
| Catalog No. | NATR-HMM-0128 |
| Description | A fluorescence-based assay kit designed for the selective and sensitive quantification of RNA in dilute samples. The kit uses an RNA-selective fluorogenic dye that minimizes interference from DNA, free nucleotides, proteins, and other common contaminants found in nucleic acid preparations. |
| Intended Use | Accurate quantitation of RNA for cDNA synthesis normalization, RT-qPCR template preparation, RNA-seq library construction, microarray labeling, and other applications requiring precise RNA input amounts. |
| Principle / Technology | An RNA-selective fluorescent dye specifically binds to RNA molecules, producing a strong fluorescence signal proportional to RNA concentration. The dye exhibits low affinity for double-stranded DNA and is unaffected by free nucleotides or proteins, ensuring that only RNA contributes to the measured fluorescence signal. |
| Detection Method | Fluorescence measurement using a dedicated fluorometer (excitation: 635–645 nm, emission: 670–680 nm for red-channel instrument); automated concentration calculation using two-point standard curve |
| Sample Type | Total RNA from any biological source (mammalian, bacterial, yeast, plant, viral); purified mRNA; in vitro transcribed RNA; RNA in common purification eluents (nuclease-free water, TE buffer, DEPC-treated solutions) |
| Performance Range / Specifications | Detection range: 0.2–100 ng per assay tube (0.025–5 ng/μL typical, depending on sample volume); linearity: R² ≥0.995; accuracy: ±10% of nominal concentration; precision: CV ≤5% for replicates |
| Sensitivity / LOD | LOD: 0.2 ng in standard assay tube; reliably detects 2-fold differences in RNA concentration; minimum quantifiable concentration: 1 ng/mL (0.1 pg/μL) with extended incubation |
| Specificity | RNA-selective fluorescence; <0.5% signal from equivalent mass of dsDNA; no interference from proteins (up to 1 μg), nucleotides (up to 1 mM), salts (up to 100 mM NaCl, 1 mM MgCl2), ethanol (up to 5%), phenol (up to 0.1%), guanidine (up to 1 mM), or common RNA extraction reagents |
| Reaction Conditions / Protocol | Dilute dye reagent 1:200 in buffer (prepare fresh), aliquot 190 μL working solution per tube, add 10 μL each standard and sample, mix, incubate 2 minutes at room temperature, read fluorescence; total assay time under 5 minutes |
| Components / Formulation | RNA HS reagent (RNA-selective fluorescent dye), RNA HS buffer, RNA standards (0 ng/μL and 100 ng/μL in TE with RNase inhibitor and preservative), assay tubes (500 included per 100-assay kit) |
| Storage Conditions | 2–8°C for reagent concentrate and standards; allow all components to equilibrate to room temperature before use; protect reagent from light; standards remain stable for 6 months after opening |
| Shelf Life | 12 months from date of manufacture at 2–8°C; protect from light |
| Package Specifications | 100 assays and 500 assays |
| Product Form | Liquid concentrate dye in DMSO storage solvent, buffer solution, RNA standards |
| Quality Control | Each lot validated: linear standard curve R² ≥0.995; accurate quantification of 2.5–1000 ng/mL RNA; specificity confirmed with dsDNA cross-reactivity <0.5%; RNase-free verified by extended incubation with sensitive RNA substrates; accuracy verified against NIST-traceable spectrophotometric standards |
| Key Features | RNA-selective dye provides accurate concentration independent of DNA contamination; 1000-fold more sensitive than A260 absorbance; unaffected by common RNA isolation reagent carryover; 5-minute total assay time; validated for Agilent Bioanalyzer and Illumina sequencing library inputs |
For research use only, not for clinical use.
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