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| Product Name | High-GC Multiplex Probe qPCR Mix with UDG |
| Catalog No. | NATR-HMM-0197 |
| Description | A multiplex probe qPCR mix optimized for GC-rich DNA templates, combining hot-start amplification, inhibitor tolerance and dUTP/UDG carryover control. |
| Intended Use | Gene-expression analysis; multiplex DNA quantification; amplification of GC-rich targets. |
| Principle / Technology | A hot-start DNA polymerase and GC-tolerant buffer promote efficient probe amplification of GC-rich templates, while dUTP/UDG reduces carryover contamination. |
| Detection Method | Multiplex fluorescent probe-based real-time PCR. |
| Sample Type | DNA templates, particularly GC-rich targets and samples containing common amplification inhibitors. |
| Performance Range / Specifications | 100 or 500 reactions; supports fast cycling, multiplex detection and robust amplification of high-GC templates. |
| Specificity | Optimized hot-start chemistry supports specific amplification and well-defined fluorescence curves. |
| Reaction Conditions / Protocol | Add primers, probes and DNA template to the 2× mix and run a compatible standard or fast multiplex qPCR program. |
| Components / Formulation | 2× high-GC multiplex probe qPCR mix containing hot-start DNA polymerase, optimized GC-tolerant buffer, dUTP/UDG system and passive-reference dye formulation. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 100 reactions; 500 reactions. |
| Product Form | 2× high-GC multiplex probe qPCR mix with UDG. |
| Key Features | High-GC template compatibility; high specificity; inhibitor tolerance; fast-program support; carryover contamination control. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Passive-reference formulation 1 is suitable for instruments such as ABI 7900HT, 7300 and StepOnePlus; formulation 2 is suitable for ABI 7500, 7500 Fast and Stratagene Mx3000P; no passive reference is required for compatible Roche and Bio-Rad instruments. |
For research use only, not for clinical use.
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