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| Product Name | Low-Background High-Sensitivity Multiplex Probe qPCR Premix |
| Catalog No. | NATR-HMM-0207 |
| Description | A 2× multiplex probe qPCR premix with low reagent-derived DNA background, high-sensitivity hot-start amplification and dUTP/UDG carryover control. |
| Intended Use | Multiplex detection of bacterial and fungal pathogens; lower respiratory tract, bloodstream and foodborne pathogen testing; sterility testing of biological products. |
| Principle / Technology | A high-affinity hot-start Taq polymerase, dual-antibody blocking and an optimized multiplex buffer support sensitive, specific amplification, while active background-DNA control and a dUTP/UDG system reduce reagent-derived and carryover contamination. |
| Detection Method | Multiplex fluorescent probe-based real-time PCR. |
| Sample Type | DNA from bacterial, fungal and other compatible pathogen-testing samples, including inhibitor-containing matrices after suitable preparation. |
| Performance Range / Specifications | 200, 500 or 2,500 reactions; 2× premix; supports fast programs completed within approximately 30 minutes; broad GC compatibility and tolerance to blood, ethanol and guanidine salts. |
| Sensitivity / LOD | Designed for high-sensitivity and low-copy multiplex detection. |
| Specificity | Strong 3′ mismatch recognition and high-efficiency hot-start blocking reduce nonspecific amplification. |
| Reaction Conditions / Protocol | Combine the 2× premix with assay-specific primers, probes and DNA template. Use a compatible standard or rapid probe-qPCR cycling program. |
| Components / Formulation | 2× multiplex probe qPCR premix containing hot-start Taq polymerase, optimized buffer, dUTP and UDG carryover-control chemistry. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 200 reactions; 500 reactions; 2,500 reactions. |
| Product Form | 2× low-background multiplex probe qPCR premix. |
| Quality Control | Reagent-background DNA, premix stability, high-sensitivity amplification, fast cycling, multiplex specificity and inhibitor tolerance are evaluated. |
| Key Features | Low reagent-derived DNA background; high sensitivity; full primer/probe premixing; approximately 30-minute fast cycling; dUTP/UDG carryover control; inhibitor tolerance. |
| Concentration | 2× premix. |
| Shipping Conditions | Ship at or below 0°C. |
| Expiration Date / Stability | After primer/probe premixing, reported performance remained stable for 7 days at 37°C, 4 months at 4°C and 60 freeze–thaw cycles. |
| Compatibility | Compatible with common multichannel real-time PCR instruments and standard or fast probe-qPCR programs. |
| Application Notes / Precautions | Use contamination-controlled workflows and assay-specific primers and probes. Include appropriate no-template and positive controls for low-copy pathogen testing. |
For research use only, not for clinical use.
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