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| Product Name | Competitive Allele-Specific Probe Genotyping Master Mix with UDG |
| Catalog No. | NATR-HMM-0200 |
| Description | A one-bottle probe-based genotyping master mix that combines competitive allele-specific amplification, dual-color fluorescence and thermolabile UDG carryover control. |
| Intended Use | SNP and InDel genotyping; plant breeding; pharmacogenomics; high-throughput sample screening. |
| Principle / Technology | Two allele-specific forward primers, one common reverse primer and two fluorescent probes generate allele-dependent FAM/HEX endpoint signals. A dUTP/thermolabile UDG system removes uracil-containing carryover products at room temperature and rapidly inactivates at 50–55°C. |
| Detection Method | Dual-color endpoint fluorescence allelic discrimination using FAM and HEX channels. |
| Sample Type | Purified or crude-extracted genomic DNA from multiple species. |
| Performance Range / Specifications | Available in 1, 5 and 25 mL formats. Reported evaluations of SNP and InDel targets showed 100% sample detection and 100% genotyping accuracy in the tested datasets; carryover-template removal exceeded 99.99% in the reported test. |
| Sensitivity / LOD | High target detection rate across tested SNP and InDel assays. |
| Specificity | Strong allele discrimination and well-separated genotype clusters for SNP and InDel loci. |
| Reaction Conditions / Protocol | Prepare allele-specific and common primers with the required reporter-tail design, combine with DNA and master mix, run PCR, and analyze terminal FAM/HEX fluorescence clusters. |
| Components / Formulation | One-bottle competitive allele-specific probe genotyping master mix with dUTP/thermolabile UDG; available in low-ROX, no-ROX and high-ROX formulations. |
| Storage Conditions | Store at −30 to −15°C protected from light. |
| Package Specifications | 1 mL; 5 mL; 25 mL. |
| Product Form | Ready-to-use probe genotyping master mix. |
| Quality Control | Detection rate, genotype accuracy, cluster separation, crude-template compatibility and carryover-removal performance are evaluated by genotyping PCR. |
| Key Features | Accurate SNP/InDel genotyping; broad crude-DNA compatibility; three passive-reference options; room-temperature carryover removal; high-throughput format. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Low-, no- and high-passive-reference formulations support different real-time PCR instrument classes and multi-species DNA samples. |
| Application Notes / Precautions | Recommended amplicon length is 45–120 bp and should not exceed 150 bp. Target-product Tm is typically 63–75°C; primer GC content is preferably 40–60%. Allele-specific forward primers require distinct 5′ reporter-tail sequences. |
| Genotype | SNP and InDel biallelic genotyping. |
For research use only, not for clinical use.
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