- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Prussian Blue Iron Stain Kit for Tissue Sections |
| Catalog No. | ATRSA-0024 |
| Description | Histochemical staining kit based on Perls' Prussian blue reaction for detection of ferric iron (Fe³⁺) in tissue sections, bone marrow aspirates, and cytology preparations. Ferric iron reacts with potassium ferrocyanide in acidic conditions to form insoluble Prussian blue pigment (ferric ferrocyanide), visible as bright blue deposits at sites of iron accumulation. Essential for hemochromatosis diagnosis and iron metabolism studies. |
| Intended Use | Histological detection of ferric iron in tissue sections; bone marrow iron store assessment (sideroblastic anemia); hemochromatosis and hemosiderosis diagnosis; iron oxide nanoparticle biodistribution studies; liver biopsy iron grading. |
| Principle / Technology | Perls' reaction: Fe³⁺ ions released by HCl react with potassium ferrocyanide to form insoluble ferric ferrocyanide (Prussian blue, Fe₄[Fe(CN)₆]₃); nuclear fast red counterstain provides contrast |
| Detection Method | Deparaffinize and hydrate sections; apply potassium ferrocyanide/HCl mixture 20-30 min; wash in distilled water; counterstain with nuclear fast red 5 min; dehydrate, clear, and mount |
| Sample Type | Formalin-fixed paraffin-embedded (FFPE) tissue sections; frozen sections; bone marrow smears; cytology preparations |
| Sensitivity / LOD | Detects ferric iron at concentrations above 0.1 µg Fe per section; visualizes hemosiderin and ferritin iron deposits |
| Specificity | Specific for ferric iron (Fe³⁺); ferrous iron (Fe²⁺) requires pre-oxidation; does not react with hemoglobin-bound iron |
| Reaction Conditions / Protocol | Staining: 20-30 min RT; counterstain: 5 min; total protocol ~45 min |
| Components / Formulation | Potassium ferrocyanide solution (5%), hydrochloric acid solution (5%), nuclear fast red counterstain (0.1%), positive control slide (hemochromatosis liver), instruction manual |
| Storage Conditions | 15-25 °C; protect from light; HCl solution store away from bases |
| Shelf Life | 24 months from manufacture date |
| Package Specifications | Kit for 50 slides, 100 slides |
| Product Form | Liquid reagents, ready-to-use |
| Key Features | Classic Perls' Prussian blue method; ready-to-use reagents; includes positive control slide; nuclear fast red counterstain; validated on automated stainers and manual protocols; results interpretable by standard brightfield microscopy |
| Purity | Potassium ferrocyanide ACS grade ≥99%; HCl ACS grade; counterstain certified by Biological Stain Commission |
| Concentration | As specified; ready-to-use or concentrate format |
| Activity / Unit Definition | Staining intensity and specificity validated on reference tissue/cell preparations |
| Molecular Weight | Varies by dye component as specified in product documentation |
| Source / Origin | Synthetic dyes and biological stains; certified by Biological Stain Commission where applicable |
| pH Range / Optimal pH | pH 6.0–7.0 for Romanowsky-type stains; varies by formulation |
| Shipping Conditions | Ambient; HCl solution is corrosive — appropriate packaging |
| Expiration Date / Stability | 24 months at 15-25 °C; discard HCl solution if cap shows corrosion; working staining solution prepare fresh — stable 2 hr |
| Regulatory / Compliance | For laboratory and research use only; RUO; HCl solution corrosive; potassium ferrocyanide not classified as dangerous at this concentration; ISO 9001 |
| Compatibility | Compatible with FFPE and frozen sections; avoid iron-containing tap water — use distilled/deionized water throughout; glassware must be acid-washed to prevent iron contamination; not for use with mercury-based fixatives (Zenker's, B-5) |
| Recommended Buffer System | Use with provided buffer or phosphate buffer pH 6.8 as specified |
| Application Notes / Precautions | Avoid contact with skin and clothing; stains are permanent; use appropriate PPE |
| Batch-to-Batch Consistency | Staining intensity and differential contrast validated on reference specimens per lot |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
