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| Product Name | Protein Quantification Assay Diluent |
| Catalog No. | ATRD-0005 |
| Description | A defined diluent for Bradford and BCA protein quantification assays, providing a blanking solution and sample diluent that eliminates interfering substance effects in total protein determination. |
| Intended Use | Diluting protein samples and preparing BSA or BGG protein standards for colorimetric total protein quantification using Bradford, BCA, and Lowry assay methods. |
| Principle / Technology | Buffer composition matches the ionic conditions of common cell lysis buffers; EDTA chelates interfering metal ions; the blank establishes the true zero for spectrophotometric measurement. |
| Detection Method | Spectrophotometric absorbance at 562 nm (BCA) or 595 nm (Bradford), standard curve linearity |
| Sample Type | Cell lysates, tissue homogenates, purified protein fractions, chromatography eluates |
| Performance Range / Specifications | Standard curve R² >0.995; compatible with 1–2000 µg/mL protein range |
| Sensitivity / LOD | N/A |
| Specificity | Compatible with RIPA, Tris-HCl, PBS, and other common lysis buffer matrices |
| Reaction Conditions / Protocol | Use as blank and sample diluent; dilute samples to fall within linear range of standard curve. |
| Components / Formulation | Same buffer composition as product-specific standards, without protein, 0.05% sodium azide |
| Storage Conditions | 2–8°C or 15–30°C depending on formulation |
| Shelf Life | 12 months |
| Package Specifications | 100 mL, 500 mL |
| Product Form | Liquid |
| Quality Control | Zero absorbance ≤0.05 at assay wavelength, standard curve verification, interference testing |
| Key Features | Matched to BCA and Bradford assays; eliminates matrix interference; ready to use; validated with multiple lysis buffer types |
For research use only, not for clinical use.
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