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| Product Name | Protein A/G Affinity Purification Column, Pre-Packed |
| Catalog No. | PTR-HMM-0082 |
| Description | A pre-packed chromatography column containing recombinant Protein A/G ligand covalently immobilized on high-performance agarose resin. This affinity matrix combines the IgG-binding domains of both Protein A and Protein G, providing broad species and subclass coverage for antibody purification from serum, ascites, cell culture supernatant, and other complex biological fluids. The column is supplied pre-equilibrated and ready for direct connection to standard FPLC and gravity-flow systems. |
| Intended Use | Affinity purification of polyclonal and monoclonal IgG antibodies from diverse sources and species using a combined Protein A/G ligand matrix. |
| Principle / Technology | Affinity chromatography with immobilized recombinant Protein A/G fusion protein |
| Detection Method | UV absorbance at 280 nm |
| Sample Type | Serum, plasma, ascites fluid, hybridoma supernatant, cell culture harvest |
| Performance Range / Specifications | Binding capacity: ≥30 mg human IgG per mL settled resin; linear flow rate: 50–300 cm/hr |
| Sensitivity / LOD | Antibody recovery: ≥85% after single-pass elution |
| Specificity | Binds IgG from human, mouse, rabbit, rat, goat, sheep, cow, and horse; minimal binding to IgM, IgA, IgE, and serum albumin |
| Reaction Conditions / Protocol | Equilibration: 5 CV binding buffer (20 mM sodium phosphate, 150 mM NaCl, pH 7.0); elution: 0.1 M glycine-HCl, pH 2.7; immediate neutralization with 1 M Tris, pH 9.0 |
| Components / Formulation | Pre-packed column containing 1 mL or 5 mL Protein A/G agarose resin, binding buffer concentrate (10×), elution buffer, neutralization buffer |
| Storage Conditions | Column: 2–8 °C in 20% ethanol; buffers: room temperature |
| Shelf Life | 18 months for column; 24 months for buffers |
| Package Specifications | 1 mL and 5 mL column sizes available |
| Product Form | Pre-packed column, ready-to-use |
| Quality Control | Each column lot tested for binding capacity and pressure tolerance; endotoxin level ≤0.1 EU/mg resin |
| Key Features | Broad species reactivity reduces the need for multiple antibody-specific purification media |
| Purity | As specified per product type; recombinant proteins ≥90% by SDS-PAGE |
| Concentration | As specified on product label or certificate of analysis |
| Activity / Unit Definition | Binding capacity or enzymatic activity as specified per product documentation |
| Molecular Weight | Varies by product as specified in documentation |
| Source / Origin | Recombinant expression in E. coli, mammalian, or insect cell systems; synthetic chemistry for small molecule reagents |
| pH Range / Optimal pH | pH 7.0–8.0 for most protein applications |
| Shipping Conditions | Ambient for dry reagents; cold packs for liquid protein solutions; dry ice for frozen products |
| Expiration Date / Stability | 12–24 months at recommended storage temperature |
| Regulatory / Compliance | Research use; manufactured under ISO 9001 quality system |
| Compatibility | Compatible with standard SDS-PAGE, Western blot, mass spectrometry, and chromatography systems |
| Recommended Buffer System | PBS, Tris, or HEPES buffers as specified in product protocol |
| Application Notes / Precautions | Avoid repeated freeze-thaw cycles for protein solutions; use fresh aliquots; protect light-sensitive reagents |
| Batch-to-Batch Consistency | Protein concentration and activity within ±15% of reference lot |
For research use only, not for clinical use.
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