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| Product Name | Protease Inhibitor Cocktail Tablets (EDTA-Free) |
| Catalog No. | PTR-HMM-0077 |
| Description | Convenient protease inhibitor tablets that dissolve rapidly in aqueous buffers to provide broad-spectrum inhibition of serine, cysteine, and metalloproteases during protein extraction and purification. The EDTA-free formulation is compatible with metal-affinity chromatography. |
| Intended Use | Broad-spectrum inhibition of endogenous proteases released during cell and tissue lysis, protecting extracted proteins from degradation for downstream applications. |
| Principle / Technology | A blend of reversible and irreversible small-molecule protease inhibitors that rapidly inactivate serine proteases, cysteine proteases, and metalloproteases upon dissolution, preventing proteolytic degradation during protein extraction. |
| Detection Method | Protease inhibition is assessed by measuring protein integrity in cell lysates via SDS-PAGE and Western blot analysis |
| Sample Type | Cell and tissue lysates from mammalian, bacterial, yeast, and plant sources |
| Performance Range / Specifications | One tablet is sufficient to inhibit proteases extracted from 10 mL of lysis buffer containing 1–5 x 10^7 mammalian cells or 1 g of tissue |
| Sensitivity / LOD | Complete inhibition of endogenous protease activity in typical cell lysates; detectable protease activity is reduced to below 1% of uninhibited levels |
| Specificity | The cocktail targets serine proteases, cysteine proteases, and metalloproteases; aspartic proteases are inhibited by a separate component in the standard (EDTA-containing) formulation; EDTA-free version excludes metalloprotease inhibition capability |
| Reaction Conditions / Protocol | Add one tablet per 10 mL of lysis buffer, crush or vortex to dissolve (2–3 minutes), add the resulting protease-inhibited buffer to cells or tissue, incubate on ice for 15–30 minutes, and proceed with protein extraction |
| Components / Formulation | Foil-sealed blister packs of 20 tablets per tube, each tablet contains a precisely formulated protease inhibitor mixture with rapid dissolution characteristics |
| Storage Conditions | 2–8°C in tightly sealed container; tablets are hygroscopic and should be protected from moisture |
| Shelf Life | 24 months when stored as recommended |
| Package Specifications | 20 tablets per tube |
| Product Form | White effervescent tablet |
| Quality Control | Protease inhibitory activity is verified for each lot using fluorogenic peptide substrates specific for each protease class; dissolution time confirmed (<3 minutes in aqueous buffer); sterility tested |
| Key Features | Tablet format eliminates the need to measure individual inhibitors and ensures consistent inhibitor concentrations across experiments; EDTA-free option is essential for metal-affinity purification protocols where chelators would strip nickel or cobalt from the resin |
For research use only, not for clinical use.
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