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| Product Name | Propidium Iodide Staining Solution, 1 mg/mL, Permeabilization Reagent Free |
| Catalog No. | RERE-0297 |
| Description | Propidium iodide stock at 1 mg/mL supplied on its own, deliberately formulated without any permeabilization reagent so that laboratories control the fixation and permeabilization chemistry themselves. PI, molecular weight 668.40, is an irritant and should be handled with gloves. The solution serves flow cytometric apoptosis and cell cycle staining and is diluted to the working strength before use. Store at -20 °C away from light; shelf life is 12 months. |
| Intended Use | Flow cytometric apoptosis and cell cycle staining where the user supplies the permeabilization step |
| Principle / Technology | PI fluoresces red after intercalating into nucleic acids of permeabilized or membrane-damaged cells |
| Specificity | 1 mg/mL; MW 668.40; no permeabilization reagent included; 12 month shelf life |
| Reaction Conditions / Protocol | Prepare cells with the permeabilization scheme of choice, add the diluted PI solution and acquire red fluorescence |
| Storage Conditions | -20 °C, protected from light |
| Package Specifications | 1 mL |
| Key Features | Reagent-only formulation leaves fixation chemistry to the user; consistent 1 mg/mL stock strength; 12 month shelf life |
For research use only, not for clinical use.
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