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| Product Name | Propidium Iodide (PI) Ready-to-Use Staining Solution |
| Catalog No. | RESK-0554 |
| Description | Propidium iodide (PI) is a commonly used nuclear fluorescent dye. As an analog of ethidium bromide (EB), it intercalates between base pairs to bind double-stranded DNA. PI is excited at 488 nm and, after a relatively large Stokes shift, reaches maximum emission at 617 nm. PI cannot penetrate intact cell membranes and is excluded from live cells, but it passes through damaged membranes to stain nuclei. Based on these properties, PI serves as a fluorescent probe for cell viability analysis. It can be used alone or together with live-cell fluorescent probes such as Calcein-AM, Hoechst 33258, or Hoechst 33342 for simultaneous staining and identification of live and dead cells, and it can be combined with 488 nm-excited fluorochromes such as FITC and PE. This product is a sterile, ready-to-use PI staining solution (10 μg/mL in PBS) that enters dead cells and binds DNA while being excluded by live cells, making it suitable for cell viability analysis by flow cytometry. |
| Intended Use | For cell viability analysis by flow cytometry; staining of dead cells with compromised membranes. |
| Principle / Technology | PI intercalates between base pairs of double-stranded DNA. It is membrane-impermeant: excluded by live cells with intact membranes, it enters only cells with damaged membranes and stains their nuclei, distinguishing dead from live cells. |
| Detection Method | Flow cytometry; Ex 488 nm, Em 617 nm (maximum). |
| Sample Type | Cells (live/dead discrimination) |
| Performance Range / Specifications | Suitable for cell viability analysis by flow cytometry. Ex/Em: 488/617 nm. Concentration: 10 μg/mL in PBS; sterile; ready to use. |
| Specificity | Binds double-stranded DNA by intercalation; excluded from live cells with intact membranes, stains dead cells with compromised membranes. |
| Components / Formulation | Sterile propidium iodide solution at 10 μg/mL in PBS; no RNase added. |
| Storage Conditions | Store at 4 °C, protected from light. |
| Package Specifications | 150 T |
| Product Form | Ready-to-use sterile liquid |
| Concentration | 10 μg/mL in PBS |
| Shipping Conditions | Shipped on wet ice |
| Expiration Date / Stability | Stable for 6 months when stored at 4 °C protected from light. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Can be used together with Calcein-AM, Hoechst 33258, or Hoechst 33342 for simultaneous live/dead staining, and combined with 488 nm-excited fluorochromes such as FITC and PE. |
| Recommended Buffer System | Supplied in PBS |
| Application Notes / Precautions | Protect from light. No RNase is added; add RNase separately if DNA-specific staining is required for the intended application. |
For research use only, not for clinical use.
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