pNPP Substrate Solution for AP-Based ELISA
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pNPP Substrate Solution for AP-Based ELISA

Cat.No: RERE-0066 Datasheet

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Product Details Related Products
Product Name pNPP Substrate Solution for AP-Based ELISA
Catalog No. RERE-0066
Description Ready-to-use 4-nitrophenyl phosphate disodium salt substrate for alkaline phosphatase. The enzyme hydrolyses pNPP to a yellow soluble product with an absorbance maximum at 405 nm, the standard readout for AP-labelled ELISA. Colour development completes within 20 minutes, and the formulation keeps background low with tight inter-batch consistency.
Intended Use AP-labelled ELISA and general alkaline phosphatase chromogenic detection.
Detection Method Alkaline phosphatase (AP) activity via pNPP hydrolysis
Performance Range / Specifications Colour development within 20 minutes; low background, stable signal and small inter-batch variation
Storage Conditions 2-8 °C protected from light
Shelf Life 1 year
Package Specifications 100 mL
Product Form Single-component liquid substrate

For research use only, not for clinical use.

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