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| Product Name | pNPP One-Component ELISA Substrate, Ultrasensitive |
| Catalog No. | RERE-0122 |
| Description | The ultrasensitive grade of the one-component pNPP substrate line, positioned for assays that must resolve trace alkaline phosphatase activity. Signal development is intensified relative to the high-sensitivity grade, lifting the low end of the response curve while the formulation retains the low background, storage stability and narrow batch variation that define the series. Supplied ready to use in a single bottle, it slots into existing AP-ELISA protocols without re-optimisation of reagent handling. |
| Intended Use | Trace-level colourimetric detection of alkaline phosphatase conjugates |
| Principle / Technology | Enhanced AP-driven p-nitrophenol generation from pNPP, measured at 405 nm |
| Sample Type | High-sensitivity AP-ELISA formats for scarce analytes |
| Specificity | One-component ready-to-use; intensified low-end response; low background |
| Reaction Conditions / Protocol | Dispense directly into wells, develop and read at 405 nm |
| Storage Conditions | 2 to 8°C |
| Package Specifications | 50 mL; 125 mL; 250 mL; 500 mL; 1 L |
| Key Features | Most sensitive grade in the pNPP range; single-bottle convenience |
| Readouts / Downstream Assays | Absorbance at 405 nm |
For research use only, not for clinical use.
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