pNPP One-Component ELISA Substrate, Standard
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pNPP One-Component ELISA Substrate, Standard

Cat.No: RERE-0123 Datasheet

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Product Details Related Products
Product Name pNPP One-Component ELISA Substrate, Standard
Catalog No. RERE-0123
Description The standard grade of the one-component pNPP substrate range, covering routine alkaline phosphatase detection in ELISA plates. Its moderate development rate suits assays with conventional incubation timing, and the ready-to-use single-bottle presentation removes any preparation variability between operators. The series characteristics of low background, stable formulation and small batch-to-batch differences carry through to this grade, making it the economical pick for high-volume routine testing.
Intended Use Routine colourimetric AP detection in high-volume ELISA workflows
Principle / Technology Standard-rate alkaline phosphatase hydrolysis of pNPP read at 405 nm
Sample Type Routine microplate AP-ELISA assays
Specificity One-component ready-to-use; conventional development rate; low background
Reaction Conditions / Protocol Add to wells, incubate for the standard development period and measure at 405 nm
Storage Conditions 2 to 8°C
Package Specifications 50 mL; 125 mL; 250 mL; 500 mL; 1 L
Key Features Economical routine grade; consistent across batches
Readouts / Downstream Assays Absorbance at 405 nm

For research use only, not for clinical use.

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