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| Product Name | pNPP One-Component ELISA Substrate, Slow Reaction Rate |
| Catalog No. | RERE-0121 |
| Description | The slow-rate member of the one-component pNPP substrate family, designed for ELISA workflows that benefit from a prolonged colour development window. Slower p-nitrophenol release gives operators more time between substrate addition and reading, which is convenient for large plate batches processed by hand and reduces the timing precision demanded of automated stations. As with the rest of the series the reagent arrives ready to use, with low background and tight batch-to-batch consistency. |
| Intended Use | Colourimetric AP detection where a long, forgiving colour development window is preferred |
| Principle / Technology | Alkaline phosphatase cleavage of pNPP at a reduced rate, read at 405 nm |
| Sample Type | Microplate AP assays, particularly manually processed large batches |
| Specificity | One-component ready-to-use; extended development window; low background |
| Reaction Conditions / Protocol | Add to wells, develop to the desired intensity and read at 405 nm |
| Storage Conditions | 2 to 8°C |
| Package Specifications | 50 mL; 125 mL; 250 mL; 500 mL; 1 L |
| Key Features | Forgiving timing for manual plate processing; consistent batch performance |
| Readouts / Downstream Assays | Absorbance at 405 nm |
For research use only, not for clinical use.
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