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| Product Name | pNPP One-Component ELISA Substrate, High Sensitivity |
| Catalog No. | RERE-0120 |
| Description | A ready-to-use single-reagent pNPP substrate tuned for high-sensitivity alkaline phosphatase detection in ELISA. Being one-component, it is poured straight into wells without dilution or buffering steps, keeping well-to-well variation low. The series is characterised by low background, stable signal development and small batch-to-batch differences, and the high-sensitivity grade gives a steeper response in the low-analyte region than the standard grade, which assists assays quantifying scarce targets. |
| Intended Use | High-sensitivity colourimetric detection of alkaline phosphatase conjugates in ELISA |
| Principle / Technology | AP hydrolysis of pNPP liberates p-nitrophenol, read colourimetrically at 405 nm |
| Sample Type | AP-conjugated ELISA systems in microplate format |
| Specificity | One-component ready-to-use format; low background; small batch-to-batch variation |
| Reaction Conditions / Protocol | Add directly to wells, incubate to develop colour and stop or read at 405 nm as required |
| Storage Conditions | 2 to 8°C |
| Package Specifications | 50 mL; 125 mL; 250 mL; 500 mL; 1 L |
| Key Features | Steeper low-end response than the standard grade; no mixing step |
| Readouts / Downstream Assays | Absorbance at 405 nm |
For research use only, not for clinical use.
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