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| Product Name | pNPP One-Component ELISA Substrate, Broad Range |
| Catalog No. | RERE-0105 |
| Description | A single-component pNPP substrate for alkaline phosphatase based ELISA measurement. The substrate formulation gives low background, stable performance and small lot-to-lot variation, with different sensitivity grades available so that the signal response can be matched to the assay; the broad range format of this listing spans a wide dynamic window, while ultra-sensitive, high-sensitivity, standard and slow variants complete the family. These characteristics suit kit development and production use where consistent colour development matters. |
| Intended Use | Colourimetric AP substrate for ELISA kit development and production |
| Principle / Technology | Alkaline phosphatase hydrolysis of pNPP releasing the yellow chromophore |
| Sample Type | AP conjugates in ELISA microwell assays |
| Specificity | Single component; low background and small lot variation; broad dynamic range format |
| Reaction Conditions / Protocol | Add the substrate to the AP containing assay, stop the reaction when developed and read the colour |
| Storage Conditions | 2 to 8°C |
| Shelf Life | 36 months |
| Package Specifications | 50 mL; 125 mL; 250 mL; 500 mL; 1 L |
| Key Features | One-component convenience with selectable sensitivity grades across the family |
| Readouts / Downstream Assays | Yellow p-nitrophenolate colour read in the ELISA reader |
For research use only, not for clinical use.
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