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| Product Name | Phosphopeptide Enrichment Magnetic Kit (TiO₂-Based) |
| Catalog No. | PTR-HMM-0083 |
| Description | A titanium dioxide-based magnetic bead enrichment system for selective isolation of phosphopeptides from complex proteolytic digests prior to mass spectrometry analysis. The enrichment principle exploits the high affinity of phosphate groups for TiO₂ surfaces under acidic conditions. This magnetic bead format simplifies the workflow by eliminating centrifugation and column steps, enabling parallel processing of multiple samples with minimal hands-on time. |
| Intended Use | Selective enrichment of phosphorylated peptides from protein digest mixtures for phosphoproteomics analysis. |
| Principle / Technology | Metal oxide affinity chromatography on magnetic TiO₂ microparticles |
| Detection Method | LC-MS/MS after enrichment |
| Sample Type | Tryptic or Lys-C peptide digests from cellular or tissue protein extracts |
| Performance Range / Specifications | Enrichment selectivity: ≥95% of captured peptides are phosphopeptides as determined by MS/MS identification |
| Sensitivity / LOD | Detection of phosphorylation sites from as little as 50 µg starting protein |
| Specificity | High affinity for mono- and multi-phosphorylated peptides; di-carboxylic acid-containing peptides are competitively displaced by optimized loading buffer |
| Reaction Conditions / Protocol | Equilibrate beads in loading buffer (80% ACN, 5% TFA, 1 M glycolic acid); bind peptides 20 min at room temperature; wash 2×; elute with 5% ammonium hydroxide, pH 10.5 |
| Components / Formulation | TiO₂ magnetic bead suspension (10 mg/mL), loading buffer concentrate, wash buffer, elution buffer, low-retention microcentrifuge tubes |
| Storage Conditions | Beads: 2–8 °C; buffers: room temperature |
| Shelf Life | 12 months for beads; 18 months for buffers |
| Package Specifications | 20 enrichments per kit (0.5 mg beads per enrichment) |
| Product Form | Magnetic bead suspension in storage buffer |
| Quality Control | Each lot functionally validated by enriching a HeLa cell tryptic digest standard; phosphopeptide enrichment rate ≥95% |
| Key Features | Magnetic format eliminates centrifugation; compatible with manual and KingFisher automated platforms |
| Purity | As specified per product type; recombinant proteins ≥90% by SDS-PAGE |
| Concentration | As specified on product label or certificate of analysis |
| Activity / Unit Definition | Binding capacity or enzymatic activity as specified per product documentation |
| Molecular Weight | Varies by product as specified in documentation |
| Source / Origin | Recombinant expression in E. coli, mammalian, or insect cell systems; synthetic chemistry for small molecule reagents |
| pH Range / Optimal pH | pH 7.0–8.0 for most protein applications |
| Shipping Conditions | Ambient for dry reagents; cold packs for liquid protein solutions; dry ice for frozen products |
| Expiration Date / Stability | 12–24 months at recommended storage temperature |
| Regulatory / Compliance | Research use; manufactured under ISO 9001 quality system |
| Compatibility | Compatible with standard SDS-PAGE, Western blot, mass spectrometry, and chromatography systems |
| Recommended Buffer System | PBS, Tris, or HEPES buffers as specified in product protocol |
| Application Notes / Precautions | Avoid repeated freeze-thaw cycles for protein solutions; use fresh aliquots; protect light-sensitive reagents |
| Batch-to-Batch Consistency | Protein concentration and activity within ±15% of reference lot |
For research use only, not for clinical use.
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