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| Product Name | Phi29 High-Processivity DNA Polymerase |
| Catalog No. | IEC-HMM-0117 |
| Description | A high-processivity Phi29 DNA polymerase with strong strand displacement and proofreading activity for isothermal DNA amplification. |
| Intended Use | Multiple-displacement amplification, whole-genome synthesis, plasmid amplification and rolling-circle amplification. |
| Principle / Technology | Phi29 DNA polymerase performs highly processive 5′→3′ DNA synthesis with strong strand displacement and 3′→5′ exonuclease proofreading. |
| Detection Method | Downstream gel electrophoresis, PCR, qPCR, sequencing or other DNA analysis. |
| Sample Type | Genomic DNA, plasmid DNA, circular DNA and compatible primed DNA templates. |
| Performance Range / Specifications | Processive DNA synthesis up to approximately 100 kb; 250 U or 1,250 U packages. |
| Specificity | Proofreading activity supports substantially higher replication fidelity than standard Taq DNA polymerase. |
| Reaction Conditions / Protocol | Combine template, primers, dNTPs, supplied reaction buffer and enzyme, then incubate under a validated isothermal amplification condition. |
| Components / Formulation | Phi29 DNA polymerase and 10× reaction buffer. |
| Storage Conditions | Store at −20°C. |
| Package Specifications | 250 U with 25 µL enzyme and 1 mL 10× buffer; 1,250 U with 125 µL enzyme and 1 mL 10× buffer. |
| Product Form | Purified enzyme solution with reaction buffer. |
| Key Features | Strong strand displacement; processivity to approximately 100 kb; 3′→5′ proofreading; high amplification fidelity; suitable for circular and genomic templates. |
| Source / Origin | Derived from Bacillus subtilis bacteriophage phi29. |
| Recommended Buffer System | Supplied 10× reaction buffer. |
| Application Notes / Precautions | Use high-purity primers and contamination-free reagents because nonspecific priming may be amplified during long isothermal reactions. |
For research use only, not for clinical use.
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