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| Product Name | PCR Reaction Sample Diluent, Nuclease-Free, with Carrier RNA |
| Catalog No. | ATRD-0023 |
| Description | Nuclease-free diluent with synthetic poly(A) carrier RNA for low-concentration nucleic acid sample dilution. Carrier RNA saturates non-specific binding sites on tube surfaces, preventing adsorption losses of low-copy-number templates. Critical for digital PCR, pathogen detection, and single-cell genomics where template loss causes false negatives. |
| Intended Use | Low-concentration DNA/RNA dilution for sensitive PCR/qPCR; prevention of adsorption losses; pathogen detection standard preparation; digital PCR sample partitioning; single-cell genomics. |
| Principle / Technology | Carrier RNA (5 ng/µL) saturates nucleic acid binding sites on polypropylene; EDTA chelation inhibits nucleases; low-EDTA minimizes PCR inhibition |
| Detection Method | Ready-to-use; dilute samples directly; use for standard curve matrix matching |
| Components / Formulation | Synthetic poly(A) carrier RNA (5 ng/µL), Tris-HCl (1 mM, pH 8.0), EDTA (0.1 mM), nuclease-free water; 0.22 µm filtered, gamma-irradiated |
| Storage Conditions | -20 °C long-term; 2-8 °C short-term (<3 months); aliquot to avoid contamination |
| Shelf Life | 24 months at -20 °C; 12 months at 4 °C |
| Package Specifications | 1 mL, 5 mL, 10 mL in LoBind tubes; 50 mL HDPE bottle |
| Product Form | Clear colorless liquid in low-bind packaging |
| Key Features | Carrier RNA recovers >90% of 10-copy templates vs. <50% with water; nuclease-free certified; low EDTA (0.1 mM); supplied in LoBind tubes; gamma-irradiated sterile |
| Purity | Synthetic carrier RNA DNase/RNase-free; human gDNA: not detected; bacterial DNA: not detected |
| Concentration | Carrier RNA 5 ng/µL ± 1 ng/µL; Tris 1 mM; EDTA 0.1 mM |
| Activity / Unit Definition | Dilution linearity R² ≥0.99 across specified range |
| Molecular Weight | Not applicable — buffered protein solution |
| Source / Origin | Bovine serum albumin, casein, and synthetic buffer components |
| pH Range / Optimal pH | pH 8.0 ± 0.1 at 25 °C |
| Shipping Conditions | Cold pack 2-8 °C; short ambient <72 hr acceptable |
| Expiration Date / Stability | 24 months at -20 °C; 12 months at 4 °C; max 5 freeze-thaw cycles |
| Regulatory / Compliance | For laboratory and research use only; RUO; synthetic carrier — no biological source; ISO 9001 |
| Compatibility | Compatible with ELISA, CLIA, and immunoturbidimetric assay platforms |
| Recommended Buffer System | PBS-based with protein stabilizers and antimicrobial preservative |
| Application Notes / Precautions | Bring to room temperature before use; avoid repeated freeze-thaw; use aseptic technique for opened bottles |
| Batch-to-Batch Consistency | Dilution recovery 90–110% for reference analyte panel per lot |
For research use only, not for clinical use.
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