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| Product Name | PCR-Grade Molecular Biology Water, DEPC-Treated and Autoclaved |
| Catalog No. | ATRD-0018 |
| Description | Ultra-pure water specifically prepared for molecular biology applications requiring the highest standards of nuclease and nucleic acid purity. The water undergoes deionization, reverse osmosis, DEPC treatment to inactivate RNases, and terminal autoclaving. It is filtered through 0.1 µm membrane and tested to confirm the absence of DNase, RNase, protease, and PCR inhibitors. Each batch is validated by qPCR for the absence of amplifiable contaminating DNA. |
| Intended Use | Resuspension and dilution of nucleic acids, preparation of PCR reactions, and enzymatic processing steps in molecular biology. |
| Principle / Technology | Multi-stage purification with DEPC-mediated RNase inactivation and terminal sterilization |
| Detection Method | qPCR negative control amplification with universal bacterial 16S rDNA primers |
| Sample Type | Purified DNA and RNA for PCR, RT-PCR, qPCR, NGS library preparation, and cloning |
| Performance Range / Specifications | Resistivity: ≥18.2 MΩ·cm at 25 °C; TOC ≤5 ppb |
| Sensitivity / LOD | No amplifiable bacterial DNA detected in 40 cycles of qPCR with universal 16S primers |
| Specificity | Free of DNase, RNase, protease, and DNA contamination as verified by functional testing |
| Reaction Conditions / Protocol | Use directly from bottle or aliquot into sterile tubes; avoid prolonged exposure to ambient air to prevent environmental nuclease contamination |
| Components / Formulation | DEPC-treated, autoclaved ultrapure water in PETG bottle |
| Storage Conditions | Room temperature; once opened, aliquot and store in small volumes to minimize contamination risk |
| Shelf Life | 18 months at room temperature in unopened bottle |
| Package Specifications | 500 mL, 1 L |
| Product Form | Liquid, clear and colorless |
| Quality Control | DNase/RNase tested by incubation with DNA/RNA substrate followed by gel electrophoresis; sterility per USP <71>; endotoxin ≤0.01 EU/mL |
| Key Features | PCR-grade water pre-qualified for absence of amplifiable DNA avoids false-positive no-template control reactions |
| Purity | Endotoxin ≤0.5 EU/mL; sterile-filtered; protein and preservative content as specified |
| Concentration | Ready-to-use; no dilution required |
| Activity / Unit Definition | Dilution linearity R² ≥0.99 across specified range |
| Molecular Weight | Not applicable — buffered protein solution |
| Source / Origin | Bovine serum albumin, casein, and synthetic buffer components |
| pH Range / Optimal pH | pH 7.2–7.6 |
| Shipping Conditions | Ambient temperature with cold packs for summer months |
| Expiration Date / Stability | 12–18 months at 2–8 °C |
| Regulatory / Compliance | For IVD use; manufactured under ISO 13485 quality system |
| Compatibility | Compatible with ELISA, CLIA, and immunoturbidimetric assay platforms |
| Recommended Buffer System | PBS-based with protein stabilizers and antimicrobial preservative |
| Application Notes / Precautions | Bring to room temperature before use; avoid repeated freeze-thaw; use aseptic technique for opened bottles |
| Batch-to-Batch Consistency | Dilution recovery 90–110% for reference analyte panel per lot |
For research use only, not for clinical use.
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