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| Product Name | Osteoblast Mineralized Nodule Staining Solution, Alizarin Red S Method |
| Catalog No. | RERE-0305 |
| Description | Purpose-built alizarin red S staining solution for visualizing calcium salts within mineralized nodules formed by cultured osteoblasts. Nodules develop a deep red color against a nearly colorless background, which makes nodule counting and mineralization area measurement straightforward in osteogenic differentiation assays. The two-bottle format covers a standard series of culture wells. Storage is 12 months at room temperature with light protection. |
| Intended Use | Demonstration of calcium salts in mineralized nodules of osteoblast cultures |
| Principle / Technology | Alizarin red S chelates the calcium salts deposited in mineralized matrix, staining nodules deep red |
| Specificity | Alizarin red S method; mineralized nodules deep red; 12 month shelf life |
| Reaction Conditions / Protocol | Fix the cultured cell layer, apply the staining solution, rinse to clear background and document the nodules |
| Storage Conditions | Room temperature, protected from light |
| Package Specifications | 2 × 50 mL |
| Key Features | Nodule-specific readout for osteogenesis assays; deep red color contrasts cleanly with background; 12 month shelf life |
For research use only, not for clinical use.
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