- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Orange-Red NHS Ester Fluorescent Labeling Dye (Ex/Em 578/603 nm) |
| Catalog No. | RERE-0006 |
| Description | This rhodamine-type dye is offered as the NHS ester for straightforward amine labelling of proteins, antibodies, peptides and nucleic acid molecules. Its extinction coefficient of 88000 delivers a strong signal, and the recommended degree of labelling of 5-6 dyes per IgG molecule gives reproducible antibody conjugates. The corrected spectral values are an excitation maximum of 578 nm and an emission maximum of 603 nm. |
| Intended Use | Amine-reactive fluorescent tagging of proteins, antibodies, peptides and nucleic acid molecules for imaging and detection. |
| Principle / Technology | The NHS ester reacts with primary amines on the target biomolecule, fixing the orange-red fluorophore through stable amide bonds. |
| Detection Method | Fluorescence detection with excitation/emission of 578/603 nm. |
| Sample Type | Red-coloured dye; no appearance form is stated on the page. |
| Performance Range / Specifications | CAS 878549-45-2; molecular formula C38H37N3O13S2; molecular weight 807.84 g/mol; Ex/Em 578/603 nm; extinction coefficient 88000; A280/Amax correction factor 0.46; optimal degree of labelling 5-6 dyes per IgG. |
| Specificity | Selective for primary amines; correction factor of 0.46 at 280 nm supports accurate protein quantification of conjugates. |
| Reaction Conditions / Protocol | Dissolve in anhydrous DMSO, react with the amine-bearing substrate at the chosen buffer conditions, then remove free dye by desalting or chromatography; target 5-6 dyes per IgG molecule for antibody work. |
| Components / Formulation | One vial of dye; solvents, buffers and purification media are not included. |
| Storage Conditions | Store at -25 to -15 °C, protected from light. |
| Shelf Life | 12 months under the recommended storage conditions. |
| Package Specifications | 1 mg or 5 mg |
| Quality Control | Spectral and protein-labelling metrics (extinction coefficient, A280 correction, optimal DOL) are stated on the page and used for release. |
| Key Features | - Extinction coefficient 88000 for a strong signal - Documented optimal degree of labelling of 5-6 dyes per IgG - A280 correction factor 0.46 for accurate conjugate quantification - Labels proteins, antibodies, peptides and nucleic acids - One-year shelf life protected from light |
| Activity / Unit Definition | Labeling performance is anchored by the published extinction coefficient and the optimal degree of labelling for IgG. |
| Molecular Weight | CAS 878549-45-2; molecular formula C38H37N3O13S2; molecular weight 807.84 g/mol. |
| Source / Origin | Synthetic small-molecule fluorophore, supplied as a purified material. |
| Expiration Date / Stability | Stability is maintained by the recommended storage temperature together with continuous protection from light. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Read-outs run on routine fluorescence equipment without proprietary hardware. |
| Recommended Buffer System | No buffer is included; prepare anhydrous stock immediately before labelling. |
| Application Notes / Precautions | Protect from light at all times, both in storage and during labelling, and avoid repeated warming cycles. Prepare stock solutions fresh where possible and confirm the instrument filter set before starting an experiment. |
| Batch-to-Batch Consistency | Lot-to-lot consistency is controlled through defined manufacturing specifications; each lot ships with its release record. |
| Model Type | Amine-reactive fluorescent dye (NHS ester) |
| Starting Material / Tissue Site | Synthesised rhodamine-type fluorophore, esterified as the NHS ester |
| Delivery Standard | Pack sizes 1 mg and 5 mg |
| Readouts / Downstream Assays | Excitation/emission 578/603 nm; protein conjugates quantified with the 0.46 A280 correction. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
