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| Product Name | One-Component TMB Membrane Substrate, Enhanced Blue-Violet |
| Catalog No. | RERE-0129 |
| Description | A precipitating one-component TMB substrate for membrane-based HRP detection that develops an enhanced blue-violet signal on the blot. The intensified colour raises band contrast against the membrane background, helping faint bands stay visible during imaging and documentation. Part of a series offering blue, violet and deep-green signal options, it serves Western blotting, line blots and dot blots in both research and in vitro diagnostic kit development, with low background and small batch-to-batch variation. |
| Intended Use | Colourimetric HRP detection on membranes for Western, line and dot blot formats |
| Principle / Technology | HRP-catalysed TMB oxidation yielding an insoluble blue-violet precipitate at the band site |
| Sample Type | Nitrocellulose and PVDF membranes probed with HRP conjugates |
| Specificity | One-component ready-to-use; enhanced blue-violet precipitating signal; low background |
| Reaction Conditions / Protocol | Incubate the membrane in substrate until bands develop, then rinse to stop |
| Storage Conditions | 2 to 8°C |
| Package Specifications | 50 mL; 125 mL; 250 mL; 500 mL; 1 L |
| Key Features | Enhanced contrast for faint bands; stable colour on membrane |
For research use only, not for clinical use.
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