One-Component TMB Membrane Substrate, Deep Blue
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One-Component TMB Membrane Substrate, Deep Blue

Cat.No: RERE-0108 Datasheet

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Product Name One-Component TMB Membrane Substrate, Deep Blue
Catalog No. RERE-0108
Description A single-component precipitating TMB substrate for membrane based detection, giving a deep blue signal on blots. The formulation shows low background, stable behaviour and small lot-to-lot variation, and the substrate family provides blue, purple and dark green signal systems for flexible selection. Typical applications are Western blot detection experiments and IVD immunoblot and line blot kits, where the precipitating colour stays on the membrane for documentation.
Intended Use Precipitating chromogenic detection on membranes for Western, line and dot blots
Principle / Technology HRP catalysed TMB oxidation precipitating the blue product on the membrane
Sample Type Membranes bearing HRP conjugated proteins after blotting
Specificity Deep blue precipitating signal; low background and small lot variation
Reaction Conditions / Protocol Cover the blot with the substrate, develop the colour and wash to stop
Storage Conditions 2 to 8°C
Shelf Life 36 months
Package Specifications 50 mL; 125 mL; 250 mL; 500 mL; 1 L
Key Features One-component membrane ready format; blue, purple and green variants in the family
Readouts / Downstream Assays Deep blue bands on the membrane

For research use only, not for clinical use.

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