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| Product Name | One-Component TMB ELISA Substrate, Slow Reaction Rate |
| Catalog No. | RERE-0125 |
| Description | A single-bottle TMB substrate whose colour development proceeds at a reduced rate, giving ELISA operators a longer interval between substrate addition and plate reading. The slower kinetics suit manually processed plate batches and laboratories that prefer not to race a fast-developing signal. Like the rest of the one-component family it needs no pre-mixing, and the formulation maintains low background with tight batch-to-batch reproducibility. |
| Intended Use | HRP-based ELISA detection where a prolonged, forgiving colour development window is preferred |
| Principle / Technology | HRP oxidation of TMB to a blue product read at 450 nm after acid stop, at a slowed development rate |
| Sample Type | Microplate HRP immunoassays processed in large or manual batches |
| Specificity | One-component ready-to-use; reduced development rate; low background |
| Reaction Conditions / Protocol | Add directly to wells, develop to the target intensity, stop with acid and read at 450 nm |
| Storage Conditions | 2 to 8°C |
| Package Specifications | 125 mL; 250 mL; 500 mL; 1 L |
| Key Features | Extended development window; no reagent mixing step |
| Readouts / Downstream Assays | Absorbance at 450 nm |
For research use only, not for clinical use.
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