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| Product Name | One-Component TMB ELISA Substrate, High Sensitivity |
| Catalog No. | RERE-0124 |
| Description | A single-reagent TMB substrate developed for high-sensitivity horseradish peroxidase detection in ELISA. The one-component presentation eliminates the mixing of chromogen and substrate solutions before use, removing a common source of well-to-well inconsistency. On reaction with peroxidase the solution develops the characteristic blue colour quantified at 450 nm after stopping, and the high-sensitivity grade sharpens the response at low analyte concentrations while holding background tight. |
| Intended Use | High-sensitivity colourimetric detection of HRP conjugates in ELISA |
| Principle / Technology | HRP-catalysed oxidation of TMB to a blue charge-transfer complex, read at 450 nm after acid stop |
| Sample Type | HRP-conjugated microplate immunoassays |
| Specificity | One-component ready-to-use; low background; sharpened low-analyte response |
| Reaction Conditions / Protocol | Add directly to wells, allow colour to develop, stop with acid and read at 450 nm |
| Storage Conditions | 2 to 8°C |
| Package Specifications | 125 mL; 250 mL; 500 mL; 1 L |
| Key Features | No pre-mixing; sensitive low-end quantification |
| Readouts / Downstream Assays | Absorbance at 450 nm |
For research use only, not for clinical use.
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