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| Product Name | One-Component TMB ELISA Substrate, Broad Range |
| Catalog No. | RERE-0107 |
| Description | A ready-to-use single-component TMB substrate for horseradish peroxidase based ELISA measurement, supplied in a broad range format that covers a wide dynamic window of enzyme concentration. In the presence of HRP the colourless TMB solution develops the characteristic blue colour that is read in a plate reader and quenched with acid stop solution for absorbance measurement. The one-component presentation removes the mixing step at the bench and suits both routine assays and kit development. |
| Intended Use | Chromogenic HRP substrate for ELISA kit development and routine plate assays |
| Principle / Technology | HRP catalysed oxidation of TMB to the blue charge transfer complex |
| Sample Type | HRP conjugates in ELISA microwell assays |
| Specificity | Single component, ready to use; broad dynamic range format |
| Reaction Conditions / Protocol | Add the substrate to the washed plate, develop the blue colour and stop with acid before reading |
| Storage Conditions | 2 to 8°C |
| Shelf Life | 36 months |
| Package Specifications | 125 mL; 250 mL; 500 mL; 1 L |
| Key Features | No mixing step; broad range format spans wide enzyme concentrations |
| Readouts / Downstream Assays | Blue colour quenched to yellow for absorbance reading |
For research use only, not for clinical use.
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