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| Product Name | Oil Red O Lipid Staining Kit, for Neutral Lipids |
| Catalog No. | ATRSA-0017 |
| Description | A complete Oil Red O staining kit for the histological demonstration of neutral lipids and triglycerides in frozen tissue sections, cultured cells, and cell smears. |
| Intended Use | Detection and visualization of lipid droplets in adipocytes and hepatocytes for fatty liver disease assessment; demonstration of lipid accumulation in cell culture models of steatosis and adipogenesis; oil droplet visualization in sebaceous glands. |
| Principle / Technology | Oil Red O is a fat-soluble diazo dye that partitions from its isopropanol solvent into neutral lipid droplets, staining them intense red; greater solubility in lipids than in solvent drives differential staining. |
| Detection Method | Bright-field light microscopy |
| Sample Type | Fresh frozen tissue sections (liver, adipose tissue, muscle), cultured cells grown on coverslips, cell smears |
| Performance Range / Specifications | Lipid droplets stained intense red; nuclei counterstained blue with hematoxylin; minimal background staining |
| Sensitivity / LOD | N/A |
| Specificity | Neutral lipids (triglycerides, cholesteryl esters) stained red; phospholipids and free fatty acids may show weak staining |
| Reaction Conditions / Protocol | Prepare frozen sections; stain in Oil Red O working solution for 10–15 min; rinse in 60% isopropanol; counterstain with hematoxylin 1–2 min; mount in aqueous mounting medium. |
| Components / Formulation | Oil Red O concentrate, isopropanol diluent, Hematoxylin counterstain, aqueous mounting medium |
| Storage Conditions | 15–30°C, tightly sealed, protect from light; prepare working solution fresh |
| Shelf Life | 24 months for stock; use working solution within 2 hours |
| Package Specifications | Kit for 100 slides |
| Product Form | Liquid concentrate with diluents |
| Quality Control | Staining validation with mouse liver (starry steatosis) and adipose tissue, lot release testing |
| Key Features | Complete kit; validated for liver steatosis studies; includes mounting medium; ready to prepare working solution |
For research use only, not for clinical use.
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